Evaluation of twelve medium components on lipase production by Bacillus sphaericus MTCC511 in submerged batch fermentation was studied using the Plackett-Burman statistical experimental design. The most significant variables affecting lipase production were found to be glucose, olive oil, peptone, NaCl and MnSO4 · H2O. Maximum lipase activity of 2.82 U ml–1 was obtained in the batch fermentatio...

A comparative study of software sensors using Multiway Partial Least Squares and Extended Kalman Filters in an application to a fed-batch yeast fermentation process is presented. The MPLS theory is introduced firstly and then applied to a yeast fed-batch fermentation process to provide soft-sensing facilities. The soft-sensing capabilities of the MPLS approach are found to compare favourably wi...

During batch fermentation, the rate of ethanol production per milligram of cell protein is maximal for a brief period early in this process and declines progressively as ethanol accumulates in the surrounding broth. Our studies demonstrate that the removal of this accumulated ethanol does not immediately restore fermentative activity, and they provide evidence that the decline in metabolic rate...

We carried out the first simulation on multi-stage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reac...

An optimal experimental design for yield coefficients estimation in an unstructured growth model of fed-batch fermentation of E. coli is presented. The feed profile is designed by optimisation of a scalar function based on the Fischer Information Matrix. A genetic algorithm is proposed as the optimisation method due to its efficiency and independence on the initial values. Copyright 2004 IFAC K...

The fermentation kinetics of penicillin production by Penicillium chrysogenum was carried out at 27°C and pH 6.0. Batch and repeated batch fermentations using agar-immobilized cells in fluidized bed bioreactor were studied for their potential application in production of penicillin from lactose, a fermentable sugar. Kinetics of immobilized cell fermentation, showed the penicillin yield of ~0.01...

This study describes an alternative mixed culture fermentation technology to anaerobically convert lignocellulosic biomass into butyric acid, a valuable product with wide application, without supplementary cellulolytic enzymes. Rice straw was soaked in 1% NaOH solution to increase digestibility. Among the tested pretreatment conditions, soaking rice straw at 50°C for 72 h removed ~66% of the li...

First report on the production of protease through fed batch cultivation using co-culture of Pseudomonas putida and Staphylococcus aureus from a crude carbon source, banana peel. Batch production by using synthetic medium, impact of some of the process parameters on cell growth and alkaline protease activity was investigated and the optimized values were found to be inoculum size of 5 % (v/v), ...

Two different types of immobilized yeast bioreactors were examined for continuous fermentation of high-gravity worts. One of these is a fluidized bed reactor (FBR) that employs porous glass beads for yeast immobilization. The second system is a loop reactor containing a porous silicon carbide cartridge (SCCR) for immobilizing the yeast cells. Although there was some residual fermentable sugar i...

Gellan gum production was carried out by Sphingomonas paucimobilis ATCC 31461 in a simplified medium with a short incubation time, and a kinetic model for understanding, controlling, and optimizing the fermentation process was proposed. The results revealed that glucose was the best carbon source and that the optimal concentration was 30 g liter(-1). As for the fermenting parameters, considerab...