The DNA is treated using the discretized wormlike bead-chain model, where each “bead” represents a 3 nm segment of relaxed DNA (1, 2). Each bead is assigned a salt-dependent charge qdna according to Stigter (3). The DNA bead-chain is also assigned an intramolecular force field comprising of stretching, bending, twisting terms that capture all aspects of DNA mechanics. The stretching energy Ustr...

Optical near-field techniques allow one to overcome diffraction by positioning an optical element in close proximity to the surface of interest. In optical trap assisted nanopatterning, this optical element is a microbead optically trapped above the substrate in a liquid environment. Using high-speed microscopy, we show that under certain conditions, the laser pulse creates a gas bubble under t...

An experimental strategy for post-eliminating thermal noise on position measurements of optically trapped particles is presented. Using a nanosecond pulsed laser, synchronized to the detection system, to exert a periodic driving force on an optically trapped 10 μm polystyrene bead, the laser pulse-bead interaction is repeated hundreds of times. Traces with the bead position following the prompt...

OBJECTIVE To compare direct and indirect bead-based T-cell isolation followed by magnetic bead-based DNA isolation. METHODS T-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead-based automated DNA isolation in 10 healthy subjects. Purity of T-cells, purity of DNA (by A260/A280 ratio measurement) and DNA concentration were assesse...

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead id...

We investigated the antitumor effects and tissue distribution of adriamycin (ADR) incorporated into a hydroxyapatite (HAP) bead in a cancer rat model bearing Swarm rat chondrosarcoma. The Porous HAP bead (8.48 mm in diameter, 531 +/- 0.7 mg in weight) was used as a model bone graft. One ADR-HAP bead (ADR 0.4 mg-6.0 mg/bead) was implanted s.c. into a Sprague-Dawley rat at 6 days postinoculation ...

[1] A technique for visualizing particle transport in the interior of a porous medium is presented. The technique, which includes the construction of a translucent medium and the use of laser-induced fluorescence for particle tracking, was used to examine the behavior of a dilute suspension of negatively charged, micron-sized particles in the interior of uniform glass bead packs during one-dime...

We used a 10-μm-diameter fluorescent bead as a test object for 3D microscopy, and independently determined its structure by examining 1-μm-thick physical sections of the bead. Images of the full bead on different 3D microscopes revealed a number of aberrations and distortions. Images also showed evidence for absorption and/or scattering on all confocal and wide-field microscopes tested, but not...

the imperialist competitive algorithm (ica) that was recently introduced has shown its good performance in optimization problems. this algorithm is inspired by competition mechanism among imperialists and colonies, in contrast to evolutionary algorithms. this paper presents optimization of bead geometry in welding process using of ica. therefore, two case studies from literature are presented t...