Size exclusion chromatography (SEC) is a widely used tool in bioseparations. Because its separation mechanism is based on the permeability of macromolecules rather than any type of binding, the feed volume to bed volume ratio is usually quite small. Thus, large columns are typically used in preparativeand large scale separations. In this work, a general rate model considering various mass trans...

Physical characteristics critical to chromatography including geometric porosity and tortuosity within the packed column were analysed based upon three dimensional reconstructions of bed structure in-situ. Image acquisition was performed using two X-ray computed tomography systems, with optimisation of column imaging performed for each sample in order to produce three dimensional representation...

This research evaluated the efficiency of combined anaerobic-aerobic processes for the treatment of slaughterhouse wastewater. The anaerobic reactor consists of a 3.95 L Plexiglas column with 60 mm diameter and 140 cm height. The cylindrical particles of polyvinyl chloride with 2 mm diameter and 1250 kg m-3 density packed to 60 cm of column were used as biomass saving material. The d...

Expanded Bed Adsorption enables the protein recovery directly from cultivations of microorganisms or cells and preparations of disrupted cells, without the need for prior removal of suspended solids. The performance ofan expanded bed is comparable to a packed bed owing to reduced mixing of the adsorbent particles, clogging/plugging of solid particles in the column, while fluidized bed requires ...

T he concept of chromatography in Expanded Bed Adsorption (EBA) was first proposed in early 90s. The initial advantage of the idea was to be able to directly process particulate containing biological feedstocks for target products, while still maintaining sufficiently high separation efficiency that is usually enjoyed only by the traditional chromatography columns. EBA could potentially replace...

The production of a modern biotechnology product, frequently a recombinant protein, requires a multistage downstream process because the feedstock is usually a liquid that is dilute in product concentration and contains many impurities some of which are unknown chemical compounds. Such a process typically centers on two or more liquid chromatography steps in order to achieve the desired purity....

Adsorption of gold cyanide on three types of Activated Carbons (ACs) has been investigated in batch and column adsorption conditions. Applied ACs have been derived from different crops precursors i.e., coconut shell (CAC), peach stone (PAC), and walnut shell (WAC). As peach stone and walnut shells are abundant agricultural residues in Iran, the activated carbons produced from these precurso...

The biosorption of copper by inactivated biomass of the brown seaweed Sargassum baccularia, immobilized onto polyvinyl alcohol (PVA) gel beads, was investigated using a laboratory-scale packed bed column. Continuous-flow column tests were performed to determine breakthrough curves at two different column lengths. In both cases, a very broad trailing edge of the breakthrough curve was observed, ...

Based on a global kinetics of direct dimethyl ether (DME) synthesis process from syngas, a steady-state one-dimensional mathematical model for the bubble column slurry reactor (BCSR) has been established. It was built on the assumption of plug flow of gas phase, sedimentation-dispersion model of catalyst grains and isothermal chamber regardless of reaction heats and rates for the design of an i...