In the present study, seven isolates of Haemophilus influenzae type b were cultured in four different media to compare growth rate and capsule production. Four liquid media namely brain heart infusion broth (BHI), trypticase soy broth (TSB), Mueller Hinton Broth (MHB) and Gonococci broth (GC) with added supplements (1% hemoglobin, 1% Isovitalex) were used. The growth was measured by colony coun...

ColorPACTM Toxin A Test is a rapid chromatographic assay for the qualitative detection of Clostridium difficile toxin A (enterotoxin) in stool specimens from patients suspected of having C.difficile-associated disease. The test can also be used for confirmation of suspect colonies of toxigenic C.difficile from agar plates or BHI broth. This assay is intended for use as an aid in the diagnosis o...

Listeria monocytogenes strain H7762, a frankfurter isolate, was tested to determine whether it was able to survive at 4 degrees C in frankfurter pack fluid (exudate) and to determine whether food exposure affects its acid sensitivity. Cultures were sampled and tested for acid sensitivity by challenge with simulated gastric fluid (SGF). SGF challenges performed immediately after inoculation reve...

This study examined the potential of Fourier transform infrared (FT-IR) absorbance spectroscopy to detect biochemical changes in bacterial cells that occur during bacterial growth phases in batch culture. Two bacterial strains, Escherichia coli ATCC 25922 and Listeria innocua ATCC 51742 were cultured in brain heart infusion (BHI) broth and incubated at 37C and cells recovered Corresponding auth...

Makanan jajanan merupakan makanan yang diolah dan dijual sebagai siap santap. disajikan harus memperhatikan sanitasi hygiene agar kualitas tetap terjaga. Penelitian ini bertujuan untuk mengidentifikasi cemaran bakteri Eschericia coli pada di Kampus Universitas Abdurrab. Bakteri Escherichia digunakan indikator mikrobiologis makanan. E. bersifat tidak patogen apabila berada dalam saluran pencerna...

The objective of this study is to investigate antimicrobial action of chlorine dioxide (ClO2) gel and hyaluronate gel (Gengigel) on dental biofilm. Pooled supra and subgingival dental biofilm were obtained from healthy individuals and incubated aerobically and anaerobically. Plaque bacteria investigated including Streptococcus constellatus, Streptococcus mitis, Eikenella corrodens, Fusobacteriu...

Francisella tularensis (Ft) is a category A biothreat agent for which there is no Food and Drug Administration-approved vaccine. Ft can survive in a variety of habitats with a remarkable ability to adapt to changing environmental conditions. Furthermore, Ft expresses distinct sets of antigens (Ags) when inside of macrophages (its in vivo host) as compared to those grown in vitro with Mueller Hi...

This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw milk and the dairy farm environment (feces, soil, feed, water) in one reaction. Brain heart infusion (BHI) broth was selected for the enrichment step to incre...

AIM The present study sought to compare the antimicrobial efficacy of 3% NaOCl, Biopure MTAD (Tulsa Dentsply, Tulsa, OK) and Brazilian ethanolic extract of propolis (EEP) against Enterococcus faecalis (E. faecalis). METHODOLOGY The study utilized 55 extracted human permanent teeth with single root canal. The samples were decoronated, instrumented and sterilized. The teeth were infected with E...

A total of 145 sewage sediments samples from municipal and industrial wastewater treatment plants were tested for the presence of Salmonella using horizontal and PCR-based methods. Samples were pre-enriched overnight in buffered peptone water (BPW) at 37°C followed by 3 h enrichment in BHI broth at 37oC. Obtained samples from BPW were selectively enriched in RVS broth (24 h, 42oC) and MKTTn (24...