نتایج جستجو برای: binding assays
تعداد نتایج: 511049 فیلتر نتایج به سال:
Markus F. Templin Dieter Stoll Monika Schrenk Petra C. Traub Christian F. Vöhringer and Thomas O. Joos* NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany. *e-mail: [email protected] The fundamental principles of miniaturized and parallelized microspot ligand-binding assays were described more than a decade ago. In the ‘ambient analy...
MATERIALS 10x Tris-glycine buffer TBE buffer may be used in place of Tris-glycine. 6x Gel-loading buffer I 32P-labeled control DNA 32P-labeled target DNA of >20 bp (specific activity 2 x 107 cpm/μg [ Labeling of the DNA fragment can be accomplished by phosphorylation (Dephosphorylation of DNA Fragments with Alkaline Phosphataseor Phosphorylating the 5' Termini of Oligonucleotides), filling in o...
The epidermal growth-factor-like domain A (EGF-A) of the low-density lipoprotein (LDL) receptor is a promising lead for therapeutic inhibition proprotein convertase subtilisin/kexin type 9 (PCSK9). However, clinical potential EGF-A limited by its suboptimal affinity PCSK9. Here, we use phage display to identify analogues with extended bioactive segments that have improved most potent analogue, ...
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