The successful application of enzymes as industrial biocatalysts requires the availability of suitable enzymes with high activity, specificity and stability in process conditions. However, naturally procreant enzymes are often not optimized to meet these requirements(Zhao et al., 2002). Using the method of directed evolution, we can not only produce useful biocatalyst for the organic chemistry ...

Pomegranate-seed residue (PSR) was used in a new strategy for the simultaneous production of Yarrowia lipolytica lipase by submerged fermentation and its immobilization adsorption. This biocatalyst—the fermented solid containing adsorbed (fermPSR)—was evaluated hydrolysis reactions structured lipid synthesis. In shake flasks, yeast extract urea were best nitrogen sources with PSR their use incr...

Ovalbumin, as the major component of egg-white, is a globular, biocompatible, nontoxic and biodegradable phosphoglyco protein. This protein with the molecular weight of 44.5 kDa, contains 385 residues of amino acids with isoelectric point (pI) of 4.5. Many purification procedures have been reported for egg-white proteins such as gel permeation and anion exchange chromatography. In this study, w...

BACKGROUND An ideal immobilized biocatalyst for the industrial-scale production of invert sugar should stably operate at elevated temperatures (60-70°C) and high sucrose concentrations (above 60%, w/v). Commercial invertase from the yeast Saccharomyces cerevisiae is thermolabile and suffers from substrate inhibition. Thermotoga maritima β-fructosidase (BfrA) is the most thermoactive and thermos...

Myoglobin has recently emerged as a promising biocatalyst for catalyzing carbene-mediated cyclopropanation, a synthetically valuable transformation not found in nature. Having naturally evolved for binding dioxygen, the carbene transferase activity of this metalloprotein is severely inhibited by it, imposing the need for strictly anaerobic conditions to conduct these reactions. In this report, ...

Key residues of Debaryomyces hansenii carbonyl reductase in the determination of the reducing activity towards aryl haloketones were identified through combinatorial mutation of conserved residues. This study provides a green and efficient biocatalyst for the synthesis of (S)-aryl halohydrins.

C1-gas conversion for sustainable synthesis of fatty acids and ethanol: dynamics CODH/AdhE1 gene expression with biocatalyst selection.