Our laboratory has characterized androgen metabolism in an androgen-responsive prostate cancer cell line (LNCaP) and showed that these cells accumulated intracellular testosterone primarily as glucuronidated metabolites. Using a cell-free assay with testosterone as substrate, we showed that LNCaP had UDP-glucuronosyltransferase (UDPGT) activity. Because dietary factors, such as flavonoids in so...

Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) is the enzyme involved in the abnormal production of the amyloidogenic peptide Aβ, one of the major causes of histological hallmarks of Alzheimer's disease (AD). Thus, BACE1 represents a key target protein in the development of new potential target for the prevention and treatment of AD. In this study, in vitro anti-AD activity of bi...

Overexpression of HER-2 receptor is associated with poor prognosis and aggressive forms of breast cancer. Scientific literature indicates a preventive role of isoflavones in cancer. Since activation of HER-2 receptor initiates growth-promoting events in cancer cells, we studied the effect of biochanin A (an isoflavone) on associated signaling events like receptor activation, downstream signalin...

Functional Feeds It is well known that the value of clover is in nitrogen. Clovers fix their own nitrogen so that an input of nitrate fertilizer is not required. Furthermore, they are rich in protein-nitrogen, and can be used to meet the protein requirements of ruminants. Clovers also contain a class of chemicals called isoflavones, and we have recently discovered evidence that the isoflavones ...

BACKGROUND Bioflavonoids are well known for their multi directional biologic activity including antidiabetic effect. It has been demonstrated that flavonoids can act as insulin secretagogue or insulin mimetic agents. OBJECTIVES This experimental study was designed in Arak University of Medical Sciences, Arak, Iran, to investigate the effects of biochanin A (a bioflavonoid) on fasting blood gl...

Non-enzymatic glycosylation of proteins is the major cause of diabetic complications, such as cardiovascular disorders, retinopathy, nephropathy and neuropathy. It seems that protein glycosylation can be inhibited effectively by antioxidants. Several flavonoids, such as rutin, kaempferol, quercetin, apigenin, naringin, morin and biochanin A were selected to determine their antioxidant effects o...

Both bifidobacteria and clostridia are part of the natural gut microflora and while clostridia may be responsible for severe intestinal infections, bifidobacteria are probiotic microorganisms belonging to the most important prospective bacteria in the bowel. The antimicrobial activity of biochanin A was tested in vitro against six Bifidobacterium spp., and eight Clostridium spp. using the broth...

I The phyto-estrogens formononet in (7-hydroxy-4 methoxylsoflavone) and biochanin A (5-7-dlhydroxy-4-methoxylsof lavone) were independently incubated in vitro at 39~ in bovine rumen fluid from a fistulated steer receiving an alfalfa hay diet. Formononet in was incubated in studies 1 and 2, whereas biochanin A was incubated in s tudy 3. The isoflavones were separated and quantified by high perfo...

This study was conducted to investigate the influence of biochanin A (isoflavone characterised by estrogenic activity) upon the growth expressed as number of cells and the content of chlorophyll a and b, total carotenoids, reducing sugars, water-soluble proteins and nucleic acids (DNA, RNA) in the unicellular green alga Chlorella vulgaris, as an experimental model. Biochanin A in a concentratio...