Phosphorus-modified phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides were chemically synthesized and their biochemical properties evaluated. Under physiological pH, these DNA analogs possess negative charge and form stable, complementary A-like DNA:RNA heteroduplexes when analyzed via circular dichroism spectroscopy. Phosphonoacetate and thiophosphonoacetate oligomers were fo...

PAI-1 is a Mr ~50,000 glycoprotein, which is the primary physiological inhibitor of the two plasminogen activators uPA and tPA. PAI-1 belongs to the serpin protein family. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta...

A total of 153 clinical isolates and 10 reference strains were employed in an investigation of CO2-dependent streptococci. Their selection was based on a lactic acid homofermentative end product. This group of organisms grew best in 5 to 10% CO2, and several species, including Streptococcus mutans, S. intermedius, Streptococcus MG, S. anginosus, and S. constellatus, required increased CO2 for p...

The kinetic mechanism and limited substrate specificity of purified terminal deoxynucleotidyltransferase from human leukemic cells is described. The reaction which utilizes two substrates, dATP as monomer, and p(dA), as initiator (where n is the chain length from 4 through 50), proceeds by a rapid equilibrium random mechanism. The polymerization reaction occurs only in the presence of a divalen...

The Onosma L. (Lithospermae, Boraginaceae) genus contains many plant species with therapeutic properties due to its rich phytochemicals. mutabilis Boiss. & Hausskn. ex (O. mutabilis) is the for which there not enough information on characteristics. Objective: total phenolic content, antioxidant activity, possible bioactive compounds, and antibacterial activities of ethanolic extracts leaf, ...