One-compartment biofuel cells without separators have been constructed, in which d-fructose dehydrogenase (FDH) from Gluconobacter sp. and laccase from Trametes sp. (TsLAC) work as catalysts of direct electron transfer (DET)-type bioelectrocatalysis in the two-electron oxidation of d-fructose and four-electron reduction of dioxygen as fuels, respectively. FDH adsorbs strongly and stably on Ketj...

The bioelectrocatalytic properties of membrane-bound aldehyde dehydrogenase (AlDH) from Gluconobacter oxydans NBRC12528 were evaluated. AlDH exhibited direct electron transfer (DET)-type activity for acetaldehyde oxidation at several kinds electrodes. kinetic and thermodynamic parameters estimated based on the partially random orientation model. Moreover, multi-walled carbon nanotube-modified e...

Enzymatic bioelectrochemistry represents the marriage of electrochemistry and enzymatic biocatalysis, has led to important applications for biosensors, biofuel cells, bioelectrocatalysis. Enzyme immobilization is basis bioelectrochemistry, as itself determines enzyme/material interface thus electrochemical performance. Amongst range methods enzyme immobilization, one-step approaches feature rap...