dilution is limited primarily in its sensitivity. However, this is of concern only if very small numbers of organisms are suspected in a particular specimen, because the lower limits of detection for a 10-μL aliquot of undiluted material would be approximately 100 cfu/mL. There exists the possibility of crossing of paths as the plates are tilted. We experienced this problem only: (i) on media t...

Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature e...