Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emis...

The number of scintillons, which are cellular organelles responsible for light emission in the marine alga Gonyaulax, were counted by both immunofluorescence and electron microscopic methods and found to vary tenfold between subjective day and subjective night. The number of scintillons peaks during the subjective night, as does stimulated bioluminescence (flashing). Furthermore, the number dro...

Firefly bioluminescence has attracted great interest because of its high quantum yield and intriguing modifiable colours. Modifications to the structure of the enzyme luciferase can change the emission colour of firefly bioluminescence, and the mechanism of the colour change has been intensively studied by biochemists, structural biologists, optical physicists, and quantum-chemistry theorists. ...

Bioluminescence imaging is a noninvasive technique whereby surface weighted images of luminescent probes within animals are used to characterize cell count and function. Traditionally, data are collected over the entire emission spectrum of the source using no filters and are used to evaluate cell count/function over the entire spectrum. Alternatively, multispectral data over several wavelength...

Bioluminescence of plankton organisms induced by water movements has long been observed and is still under investigations because of its great complexity. In particular, the exact mechanism occurring at the level of the cell has not been yet fully understood. This work is devoted to the study of the bioluminescence of the dinoflagellates plankton species Pyrocystis noctiluca in response to mech...

In preclinical cancer research, bioluminescence imaging with firefly luciferase and D-luciferin has become a standard to monitor biological processes both in vitro and in vivo. However, the emission maximum (λmax) of bioluminescence produced by D-luciferin is 562 nm where light is not highly penetrable in biological tissues. This emphasizes a need for developing a red-shifted bioluminescence im...

Bioluminescence—i.e., the emission of visible light by living organisms—is defined as a biochemical reaction involving, at least, luciferin substrate, an oxygen derivative, and specialised luciferase enzyme. In some cases, enzyme substrate are durably associated form photoprotein. While this terminology is educatively useful to explain bioluminescence, it gives false idea that all luminous orga...

The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (≤250 ...

abstract the spontaneous emission spectrum from a driven three-level atom placed inside a double-band photonic crystal has been investigated. we use the model which assumes the upper levels of the atomic transition are coupled via a classical driving field. the transition from one of the upper levels to lower level couples to the modes of the modified reservoir, and the transition from the othe...