Recent papers have described new bioreactor designs. Most innovations addressed either oxygen transfer, shear induced by stirring, control of water activity in organic phase systems or waste biotreatment. Innovations made during the past year were reported in mainly three areas: bioreactor designs for increases in oxygen transfer and decreases in shear stress; bioreactors for two-phase reaction...

Adoptive cell transfer immunotherapy against melanoma is highly effective. However, this therapy has seen limited dissemination, mainly due to the complexity and costs of cell expansion protocols. Two bioreactors have recently been described that simplify and streamline the production of individualized cell therapies. Such bioreactors might increase the number of patients that get access to thi...

The low-shear, microgravity-simulating cell culture environment in Rotating Wall Vessel (RWV) Bioreactors RWV Bioreactors is well suited for generating differentiated 3-D tissue constructs (organoids). Biochemical, immunological and molecular biological techniques, including cDNA arrays, were used to analyze signal transduction, catecholamine contents, and gene expression in 3-D tissue-like con...

S ingle-use bioprocessing equipment has come to thoroughly dominate precommercial biopharmaceutical production in only a decade. Yet even with this breakthrough, performance and cost pressures on biopharmaceutical facilities continue to grow. Demands for greater productivity, more efficiency, and lower costs are resulting in an unrelenting push for upstream improvements. Some people in the indu...

The aim of this study was to determine the reproducibility of small volume repeat sampling from replicate bioreactors with stabilized continuous-flow chicken cecal bacterial communities. Bacterial concentration and diversity were analyzed by phenotypic, biochemical and ribotype analysis. Significant differences in concentrations and variations in diversity were found in replicate bioreactors.

the mixing behavior of the gas-liquid phase in three loop bioreactors was investigated. a gas-induced external airlift loop bioreactor (ealb), a forced-liquid vertical tubular loop bioreactor (vtlb) and a forced-liquid horizontal tubular loop bioreactor (htlb) were used for mixing studies as well as biomass production from natural gas. the effect of design parameters, riser to downcomer cross s...

BACKGROUND Single-use rocking-motion-type bag bioreactors provide advantages compared to standard stirred tank bioreactors by decreased contamination risks, reduction of cleaning and sterilization time, lower investment costs, and simple and cheaper validation. Currently, they are widely used for cell cultures although their use for small and medium scale production of recombinant proteins with...