The enzyme cystathionase from rat liver is inactivated by incubation with 5,5’-dithiobis(Z-nitrobenzoic acid) at pH 7.4. The reaction of four -SH groups per mole of enzyme brings about 95% loss of the homoserine deaminase activity. Kinetically, the reactive --SH groups can be classified into two classes. The substrate L-homoserine or the competitive inhibitor L-alanine has no effect on the rate...

The enzyme cystathionase from rat liver is inactivated by incubation with 5,5’-dithiobis(Z-nitrobenzoic acid) at pH 7.4. The reaction of four -SH groups per mole of enzyme brings about 95% loss of the homoserine deaminase activity. Kinetically, the reactive --SH groups can be classified into two classes. The substrate L-homoserine or the competitive inhibitor L-alanine has no effect on the rate...

To develop a fluorescent ruthenium complex for biosensing, we synthesized a novel sulfhydryl-reactive compound, 4-bromophenanthroline bis-2,2'-dipyridine Ruthenium bis (hexafluorophosphate). The synthesized Ru(II) complex was crosslinked with thiol-modified protein G to form a universal reagent for fluorescent immunoassays. The resulting Ru(II)-protein G conjugates were identified by sodium dod...

Gold nanoparticles synthesized via sodium citrate reduction of chloroauric acid (HAuCl(4)) were functionalized with either various concentrations of thiol-terminated Bodipy(®) FL L-cystine (0.5, 1.0, 1.5, and 2.0 μg/mL) or Bodipy-poly(ethylene glycol) at concentrations of 0.5-18.75, 1.0-12.50, and 1.5-6.25 μg/mL to form a mixed monolayer of BODIPY-PEG. Thiol-terminated Bodipy, a fluorescing mol...

Thiol-ene photopolymerizations gain a growing interest in academic research. Coatings and dental restoratives are interesting applications for thiol-ene photopolymerizations due to their unique features. In most studies the relative flexible and hydrophilic ester derivative, namely pentaerythritoltetra(3-mercaptopropionate) (PETMP), is investigated as the thiol component. Thus, in the present s...

The systemic use of thiol-containing uroepithelial protecting agents, e.g., N-acetylcysteine (NAC) or mesna, in conjunction with the alkylating agent cyclophosphamide is predicated on the assumption that the toxic metabolic by-products will be consumed by thiol without diminishing the cytotoxicity of the active alkylating intermediate, phosphoramide mustard. Studies in murine tumor systems have...

Our previous in vitro studies demonstrated marked synergy with alkylating agents when novobiocin was present during and after alkylating agent exposure. To determine whether this effect is observed in vivo, novobiocin was administered daily for 3 days prior to alkylating agent treatment, during alkylating agent treatment, and for 2 days after completion of alkylating agent treatment. When combi...

Sulfhydryl-containing compounds, including thiols and hydrogen sulfide (H2S), play important but differential roles in biological structure and function. One major challenge in separating the biological roles of thiols and H2S is developing tools to effectively separate the reactivity of these sulfhydryl-containing compounds. To address this challenge, we report the differential responses of co...

Our previous in vitro studies demonstrated marked synergy with alkylating agents when novobiocin was present during and after alkylating agent exposure. To determine whether this effect is observed in vivo, novobiocin was administered daily for 3 days prior to alkylating agent treatment, during alkylating agent treatment, and for 2 days after com pletion of alkylating agent treatment. When comb...