Carbapenemases production, either metallo-lactamases (MBLs), KPC or oxacillinases (OXA), is the main resistance mechanism responsible for resistance phenotype to carbapenems in Acinetobacter.1 While for MBLs and KPCs there are some screening tests for their detection,2,3 the same is not true for oxacillinases. During the investigation of a large outbreak, we analyzed 584 carbapenem-resistant Ac...

Acinetobacter pittii 44551 was recovered from a patient with gout combined with tuberculosis and was found to harbor the carbapenemase genes blaNDM-1 and blaOXA-58 on two different plasmids pNDM-44551 and pOXA58-44551, respectively. pNDM-44551 displayed high self-transferability across multiple bacterial species, while pOXA58-44551 was likely co-transferable with pNDM-44551 into A. baumannii re...

The carbapenem-hydrolyzing class D β-lactamase OXA-253 was identified in an Acinetobacter baumannii clinical isolate belonging to sequence type 113 (ST113) in Brazil. OXA-253 shares 93.8% amino acid identity with OXA-143. The blaOXA-253 gene is located on a ca. 20-kb plasmid. The genetic environment of the blaOXA-253 gene shares the highest identity with ubiquitous GR2 group plasmids usually ca...

Sir, The emergence and dissemination of Klebsiella pneumoniae isolates harbouring carbapenemases is a serious problem. Since the initial report of the OXA-48 enzyme in a K. pneumoniae isolate from Turkey in 2001, OXA-48 producers have been reported in many countries of the world. – 4 Current reports indicate that OXA-48 producers are widespread, mostly from Mediterranean countries as well as ot...

From a total of 320 bacterial samples from wound swab and urine 169 (53%) gram-negative bacteria were isolated, of which 42 (25%) extended-spectrum beta-lactamase (ESBL) producers were detected by double-disk synergy test. ESBL producers were significantly more resistant against amoxiclav, Co-trimoxazole, ciprofloxacin, amikacin and gentamicin than non-ESBL producers. Among the 42 ESBL producer...

Sir, Acinetobacter bereziniae, previously known as Acinetobacter genomic species 10, has been isolated primarily from clinical specimens and the hospital environment and more rarely from various other sources, including vegetables, soil and animals. Antibiotic resistance is rarely reported in this species. Over the last decade, carbapenem resistance in Acinetobacter spp., mainly Acinetobacter b...

INTRODUCTION Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. baumannii isolated from a Spanish hospital in term...

Oxacillinase (OXA)-48-like carbapenemases remain relatively uncommon in the United States. We performed phenotypic and genotypic characterization of 30 Enterobacteriaceae producing OXA-48-like carbapenemases that were recovered from patients during 2010-2014. Isolates were collected from 12 states and not associated with outbreaks, although we could not exclude limited local transmission. The a...

OBJECTIVES To obtain the complete genome sequence of an Acinetobacter johnsonii isolate, which encodes the NDM-1 and OXA-58 carbapenemases. METHODS Genome sequencing was performed using the 454 and HiSeq 2000 platforms. Reads were assembled into scaffolds and gaps between scaffolds were filled by PCR and Sanger sequencing. Phylogenetic analyses of A. johnsonii isolates and their intrinsic bla...

Sir, Carbapenem resistance in Acinetobacter baumannii is now increasingly reported. The most prevalent mechanism of carbapenem resistance in A. baumannii corresponds to the production of acquired carbapenem-hydrolysing class D b-lactamases (CHDLs) encoded by blaOXA-23-like, blaOXA-40-like or blaOXA-58-like genes. Whereas the blaOXA-40 gene has been detected in Portugal, Spain and the USA, blaOX...