OBJECTIVES VIM-type enzymes are among the most widespread acquired metallo-β-lactamases among Gram-negative pathogens. Integron In70 is a class 1 integron that has emerged as a successful genetic support for blaVIM-1 (one of the most prevalent blaVIM allelic variants) in Gram-negative non-fermenters, and is usually chromosome borne. The objective of this study was to characterize plasmid pAX22 ...

To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to detect and differentiate between the most prevalent carbapenemase genes encountered in Enterobacteriaceae (bl...

Introduction: Enterobacter cloacae strains have been isolated from Eastern Cape hospitalised patients. Methodology: We have molecularly characterised blaOXA-48-, blaIMPand blaVIM-expressing E. cloacae isolates demonstrating resistance to carbapenems from five hospitals by multilocus sequence typing. Organism identification and antimicrobial susceptibility testing was done using automated system...

Acquired metallo-lactamases (MBLs) are emerging resistance determinants of great clinical concern. They are found in Enterobacteriaceae, Pseudomonas aeruginosa, and other Gram-negative nonfermenters, in which they can confer a very broad spectrum of -lactam resistance, including the expanded-spectrum cephalosporins and carbapenems, that cannot be reverted by -lactamase inhibitors currently avai...

Carbapenems, mainly imipenem and meropenem, are potent agents for the treatment of infections due to multiresistant Pseudomonas sp. clinical isolates. However, the prevalence of carbapenem resistance in this genus has been increasing worldwide. High-level resistance to carbapenems ( 32 g/ml) is still uncommon in Pseudomonas spp. but can be due to the presence of Ambler class B -lactamases—metal...

A Pseudomonas aeruginosa é uma bactéria oportunista, considerada um dos principais agentes de infecção nosocomial em hospitais associada múltipla resistência a antimicrobianos. Em animais, principalmente cães, otite enfermidade grande incidência associado P. aeruginosa. ocorrência isolados multirresistentes metalo beta lactamase com presença genes blaIMP-1, blaVIM-2, blaSPM-1 e blaNDM-1 tem sid...

Background: Diabetic foot infection (DFI) is an in soft tissue or bone patient with diabetes mellitus, Citrobacter species are infrequent nosocomial pathogens and cause a range of infections. the aim current study we report isolation carbapenem genes among isolated from DFI. Methods: about 111 swabs specimen were collection suspected DFI during period study, determine antibiotic susceptibility ...

We investigated the presence of carbapenemases in carbapenem-resistant Pseudomonas aeruginosa isolates, which were collected over a 14-month period Turkish hospital, with in-depth molecular characterization carbapenemase-producing isolates. Among 45 study 2 isolates identified as carbapenemase producers by both Carba NP and Carbapenem Inactivation Method tests, only 1 them gave positive result ...

pseudomonas aeruginosa is among the most important pathogens in the nosocomial infections. a genetic mobile element, the integron, is one of the major agents involved in dissemination of multi-drug resistance among gram negative bacteria. during a descriptive study from october 2009 to august 2010, some 130 p. aeruginosa clinical isolates were collected from different wards of three hospitals i...