The Galleria mellonella infection model was used to assess the in vivo efficacy of phage therapy against laboratory and clinical strains of Pseudomonas aeruginosa. In a first series of experiments, Galleria were infected with the laboratory strain P. aeruginosa PAO1 and were treated with varying multiplicity of infection (MOI) of phages either 2h post-infection (treatment) or 2h pre-infection (...

lamerin microspridia (lbms) isolated from lamerin breed of the silkworm, bombyx mori, l. and the standard strain nosema bombycis, were induced to germinate artificially by two-step procedure. fresh percoll purified spores were activated by incubating them in potassium hydroxide solution (koh) (ph 11.00) and germinated artificially by using phosphate buffer saline (pbs), (ph 7.00). germinated sp...

As a first step in evaluating the tumor suppressor activity of the manganese superoxide dismutase (MnSOD) gene on established tumors in vivo, we used adenovirus-mediated gene transfer as a means of delivering the MnSOD cDNA to hamster cheek pouch carcinoma (HCPC-1) cells in vitro. HCPC-1 cells were transduced with the adenovirus-MnSOD construct (AdMnSOD) at multiplicities of infectivity (MOI) o...

Helicobacter pylori infection of the human stomach causes chronic gastritis that can lead to gastric cancer. Because activated lymphocytes persist in the gastric mucosa, and because a high multiplicity of infection (MOI) of H. pylori is needed to induce apoptosis in vitro, we speculated that resistance of lymphocytes to apoptosis is an important feature of the immune response to H. pylori. Fres...

A cross-sectional survey was done to assess the missed opportunity for immunization (MOI) in children under two years of age attending Medical Outpatient, Newborn Follow-up Service and Immunization Clinic of Institute of Child Health and to evaluate interventions. Baseline survey phase-I was done and two interventions: (i) education and awareness of immunization among health personnel; and (ii)...

INTRODUCTION TO THE PROBLEM Muhimbili Orthopaedic Institute (MOI) is a public, academic tertiary care referral center in Dar es Salaam, Tanzania, East Africa. MOI is the main referral center for orthopedic and neurosurgical patients in Tanzania. In July 2014, the Radiology Department at MOI consisted of two attending radiologists, 10 technologists, and three IT staff with imaging capabilities o...