An enzyme immunoassay that uses easily regenerated reagents was developed and evaluated for the ability to detect group B rotaviruses (GBR) in fecal specimens. Homologous rat GBR and heterologous porcine and bovine GBR were detected by this immunoassay, although a human GBR isolate was not. This immunoassay should prove useful in studies of GBR infections of animals.

By sequence and phylogenetic analyses, the 11 genomic segments of two bovine rotaviruses isolated from clinically infected calves were proven to be derived from the swine-like P[7]G5 genotype. This finding reinforced the hypothesis that interspecies transmission of completely heterologous strains can occur in nature.

Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplified fragments-three of them of animal origin (porcine or bovine) and one of huma...

A total of 83 faecal samples carrying rotavirus were used in this survey. Five different electrophoretypes were distinguished according to their migration patterns. All the rotaviruses examined in this research proved to be group A and to have short migration patterns. While local migration patterns were detected in individual herds (e.g. A, B, C, M, P and T), two different migration profiles w...

Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55.7% of the samples; viruses of the G10 and G8 serotypes were found in 34.9 and ...

A literature review was made to assess the epidemiology, public health importance, diagnostic and control methods of bovine rotavirus. Rotavirus is genus name under family Reoviridae which characterized by segmented genome. The emergence new serotypes virus related nature viral genome allows reassortment during mixed infections. rotavirus consists 11 double-stranded RNA gene segments encoding 6...

Accepted: 01/05/2015 Abstract Group A rotaviruses play an important role in causing gastroenteritis and mortality in buffalo calves. To investigate the epidemical characteristics and prevalence of bovine rotavirus (Group A rotavirus) in Punjab, 120 fecal samples were collected from buffalo calves (n=93) and cow calves (n=27) up to 3 months age group calves exhibiting diarrhoea during the period...