Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with requ...

Zeolite micropattern created from structurally similar hydrophobic Sil-1 and hydrophilic ZSM-5 zeolites, and tested for the immobilization of fluorescein isothiocyanate conjugate bovine serum albumin (BSA-FITC), biotin conjugate bovine serum albumin (Biotin-BSA), Hochest 33342 and rhodamine conjugate αbungatoxin, were preferentially deposited onto hydrophobic ZSM-5 patterns regardless of the pa...

The lipid A component of bacterial lipopolysaccharides (endotoxins), when complexed to bovine serum albumin (BSA) or human serum albumin (HSA), was shown to be a potent pyrogen. Furthermore, rabbits could be protected against endotoxin fever by immunization with both lipid A BSA and lipid A HSA complexes. The results presented in this paper show that lipid A is responsible for the pyrogenic act...

Epicatechin (EC) was used in this study to antagonize the cognitive dysfunction caused by lead (Pb) exposure mice. Eight-week-old male Kunming mice were treated with PbCl2 (20 mg/kg) and/or EC (50 gavage administration for 4 weeks. Morris water maze test showed that could improve memory induced Pb. antagonized Ca2+ overload, activated Nrf2 signaling pathway and reduced acc...

Dietary flavonoids show beneficial effects in the prevention of chronic diseases. However, flavonoid bioavailability is poor, probably due to their interaction with serum albumins. In the current work, the binding interactions of eight related flavonoids, sharing a similar core structure, with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. The binding affinities of t...

A quantitative method was developed for the determination of proteins in aqueous solution. Under optimal conditions, the linear ranges of calibration curves for the determination of bovine serum albumin (BSA), human serum albumin (HSA) and egg albumin (Alb) were 01.13 μg ml-1, 0-1.17μg ml-1 and 0-1.13μg ml-1, respectively. The detection limits were 15.74 ng ml-1 for BSA, 23.34 ng ml-1 for HSA a...

Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) in combination with electrochemistry, was employed to study the influence of surface charge (potential) on the kinetics of bovine serum albumin (BSA) adsorption on a biomedical-grade 316LVM stainless steel surface is discussed. The BSA adsorption kinetics was found to greatly depend on the surface potential. With an ...

Serum albumins are the most abundant carrier protein in the circulatory system and are involved in the transportation and distribution of exogenous and endogenous materials in blood [1], including nutrients and drugs, mostly by the formation of noncovalent complexes at binding sites [2]. The absorption, distribution, metabolism, and excretion properties as well as the stability and toxicity of ...

The equilibrium adsorption of bovine serum albumin (BSA) on poly-3-hydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) surfaces was studied at different solution concentrations. data were fitted to the Langmuir Freundlich models. protein behaviour further investigated by analysing conformation surface adsorbed BSA using Fourier transform infrared (FTIR) spectroscopy wi...

This study aimed to investigate the effect of Gadolinium oxide (Gd2O3) concentration (0.5%, 1%, and 2%) on performance polysulfone (PSF) membrane. The membranes were fabricated by a common phase inversion method. was determined based pure water flux, bovine serum albumin (BSA) permeation test. These characterized FTIR contact angle measurements. highest BSA rejection 53%. optimum membrane (2 % ...