Recently, bromodeoxyuridine (BrdU) has been successfully applied to the measurement of bacterial productivity as an alternative to tritiated thymidine (3H-TdR), which is widely used but often restricted by regulations, particularly in field settings. Here, I report improvements to existing BrdU methods to simplify procedures and increase sensitivity. The feasibility of the method was tested mea...

Bromodeoxyuridine (BrdU) immunohistochemistry is the method of choice for labeling newly generated cells in the brain. Most BrdU studies utilize paraformaldehyde-fixed brain tissue because of its compatibility with both BrdU and other immunohistochemical methods. However, stronger fixation is required for electron microscopic studies, and unfixed tissue is needed for biochemical and molecular s...

RATIONALE The ability of the adult heart to generate new myocytes after injury is not established. OBJECTIVE Our purpose was to determine whether the adult heart has the capacity to generate new myocytes after injury, and to gain insight into their source. METHODS AND RESULTS Cardiac injury was induced in the adult feline heart by infusing isoproterenol (ISO) for 10 days via minipumps, and ...

Ueda, Junji, Hiroshi Saito, Hiroaki Watanabe, and B. Mark Evers. Novel and quantitative DNA dot-blotting method for assessment of in vivo proliferation. Am J Physiol Gastrointest Liver Physiol 288: G842–G847, 2005. First published November 11, 2004; doi: 10.1152/ajpgi.00463.2004.—Immunohistochemical assessment of 5-bromo-2-deoxyuridine (BrdU) in tissue sections is a widely used method to evalua...

PURPOSE Recent production of a monoclonal antibody, PC10, against proliferating cell nuclear antigen (PCNA) makes it possible to evaluate cell cycling in formalin-fixed tissues. In this study, the authors quantitatively evaluated the relationship between PCNA expression and two other measures of cell cycling, bromodeoxyuridine labeling index (BrdU LI) and mitotic index (MI), in archival uveal m...

5-Bromo-2-deoxyuridine (BrdU) is a thymidine analogue that is incorporated into replicating DNA. Although originally designed as a chemotherapeutic agent, sublethal concentrations of BrdU have long been known to alter the growth and phenotype of a wide range of cell types. Mechanisms underlying these BrdU-mediated effects remain unknown, however. We have characterized the effects of BrdU on A54...

Mechanisms regulating masculinization of the zebra finch song system are unclear; both estradiol and sex-specific genes may be important. This study was designed to investigate relationships between estrogen and ribosomal proteins (RPL17 and RPL37; sex-linked genes) that exhibit greater expression in song control nuclei in juvenile males than females. Four studies on zebra finches were conducte...

Several reports have indicated that prolactin-secreting cells (PRL cells) are generated from growth hormone-secreting cells (GH cells). We have shown that treatment with a combination of epidermal growth factor (EGF), insulin, and estradiol-17beta (E (2)) induces the appearance of PRL cells in pituitary tumor GH3 cells. The aim of the present study was to clarify the involvement of mitosis in t...

Na krševitom brdu Okešar, nadomak Podgorice, zamišljen je park prirode sa eko domaćin­stvom: objektima stočarstva; poljoprivrede; smještaja i ugostiteljstva. Analizirani su prirodni potencijali kraškog predjela Crne Gore na primjeru lokacije donešeni zak­ljučci o strategiji transformacije neiskorišćenih kraških ekološki ekonomski isplativ način.

Recent studies suggest that gastrin regulates parietal cell maturation. We asked whether it also regulates parietal cell life span and migration along the gland. Dividing cells were labeled with 5'-bromo-2'-deoxyuridine (BrdU), and parietal cells were identified by staining with Dolichos biflorus lectin. Cells positive for D. biflorus lectin and BrdU were reliably identified 10-30 days after Br...