the effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mosml) and two levels of glycerol (5 and 7 %) .in general, semen samples were collected 36 times (from e...

The attempt to achieved the goal could be through using artificial insemination (AI) by frozen semen. This study aims evaluate effect of breed on sperm motility Madura, Bali bulls and Bos taurus experiment used local bull semen (Madura breeds) (Simmental Limousin produced Singosari’s center. Sperm evaluation was conducted computer-assisted analysis (CASA, Spermvision, Minitube®, Germany). analy...

The present study evaluated the relationship between Bali bull (Bos javanicus) seminal plasma proteins and different semen quality parameters. Semen samples from 10 mature Bali bulls were evaluated for conventional semen parameters (general motility, viability, and normal morphology), sperm functionality (acrosome reaction, sperm penetration rate, sperm penetration index), sperm kinetics (compu...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation | Yotsapon Yangnam, Suttiwat Chapanya, Thevin Vongpralub, Wuttigrai Boonkum, Vibuntita Chankitisakul Agricultural Journals

Male fertility, the ability of sperm to fertilize and activate the egg and support early embryogenesis, is vital for mammalian reproduction. Despite producing adequate numbers of sperm with normal motility and morphology, some males suffer from low fertility whose molecular mechanisms are not known. The objective was to determine apoptosis in sperm from high and low fertility bulls and its rela...

The present study aimed to establish an efficient system for bovine embryo production by in vitro fertilization (IVF) that can achieve stable normal fertilization and blastocyst developmental rates in any bull without optimization of the sperm concentration in IVF medium. We examined the effects of a PHE mixture (20 μM D-penicillamine, 10 μM hypotaurine and 1 μM epinephrine), theophylline (2.5 ...

BACKGROUND Control of the medium osmolarity and temperature during long or short time sperm manipulation is essential. OBJECTIVE The objectives of the present study were to find the effects of different osmolarities of modified Tyrode's solution and milk on the bull sperm during incubation at above zero temperatures. MATERIALS AND METHODS Semen samples were collected twice from five Najdi b...

Mammalian spermatozoa expend energy, generated as intracellular ATP, largely on motility. If the sperm cell cannot swim by use of its flagellar motion, it cannot fertilize the egg. Studies of the means by which this energy is generated span a period of six decades. This review gives an overview of these studies, which demonstrate that both mitochondrial oxidative phosphorylation, for which oxyg...