GB virus C (GBV-C; also called hepatitis G virus) is a common cause of infection associated with prolonged survival among HIV-infected individuals. The prevalences of GBV-C viremia vary widely in different studies, and there has been poor agreement among different laboratories performing GBV-C RNA detection in quality control studies. To determine the optimal method of measuring GBV-C RNA in cl...

AIM OF THE STUDY Association of two HLA class I variants with HIV-1 pretreatment viremia, CD4+ T cell count at the care-entry and CD4+ T cell nadir. METHODS 414 HIV-positive Caucasians (30% women) aged 19-73 years were genotyped for HLA-C -35 (rs9264942) and HLA-B*5701 variants. HIV-1 viral load, as well as CD4+ T cell count at care-entry and nadir, were compared across alleles, genotypes and...

BACKGROUND Hepatitis C virus infection is now one the common infection in Pakistan. Patients are routinely screened by antibody assays. Objective of this study was to assess the viremia in patients labelled as anti-HCV positive by ELISA. METHODS It this retrospective study patients labelled as anti HCV positive by ELISA were assessed for HCV RNA by polymerase chain reaction. The 254 HCV posit...

of age. Sex was not significantly associated (mean male to female sex ratio was 32.4:18 for S. typhi and 15.8:10.6 for S. paratyphi A). S. typhi has become increasingly sensitive to amoxycillin, chloramphenicol, and gentamicin, increasing from 75.1% in 1994 to 96.6% in 1998 for amoxycillin, from 71.9% in 1994 to 91.6% in 1998 for chloramphenicol, and from 96.4% to 100% for gentamicin. S. paraty...

The present study aimed at standardizing a real-time quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV RNA. A "TaqMan" assay using primers and probe derived from the 5¢ NCR region was developed and validated. Two hundred and fifty-three plasma samples from HIV-infected women were tested for GBV-C viremia and antibody against the envelope protein 2. GBV-C RNA was...

Hepatitis C virus (HCV) is the major etiologic agent associated with non-A, non-B hepatitis. This study was designed to assess virologic and serologic markers in hemophiliacs exposed to non-heat-treated and/or virus-inactivated plasma derivatives. Serial bleeds from 48 hemophilic patients were analyzed for the presence of HCV viral RNA sequences as detected by polymerase chain reaction (PCR) an...

Background/Aim. Quantitative hepatitis B surface antigen (qHBsAg) has become increasingly widespread in the last few years both diagnostic and therapeutic protocols for chronic HBV infection. Numerous studies have proposed it as a surrogate marker covalently closed circular DNA (cccDNA). Our study aimed to examine correlation between qHBsAg viremia untreated patients. Methods. The included 112 ...

Acute hepatitis C virus (HCV) is typically defined as new viremia and antibody seroconversion. Rates and immunologic correlates of hepatitis C clearance have therefore been based on clearance of viremia only in individuals who initially had an antibody response. We sought to characterize the immunological correlates of clearance in patients with acute hepatitis C and their sexual contacts. We p...