Tamm-Horsfall glycoprotein was isolated from hamster urine, and antiserum against it was produced in rabbits. IgG was isolated from the antiserum. Immunocytochemical methods were used to localize Tamm-Horsfall-like immunoreactivity in three substrains of baby-hamster kidney (BHK) cells. Indirect immunofluorescence techniques showed that, in two substrains (BHK-21/C13/2P and BHK-21/C13/3P), a pr...

Introduction: In vivo and non-invasive measures of hepatic and muscular glycogen are needed to effectively study diabetes and metabolism in humans and animals. C13-MRS methods have been shown to accurately measure glycogen levels; however, C13-MRS suffers from relatively poor spatial resolution and is not available on the majority of clinical MRI scanners. More recently, glycogen’s inherent CES...

A convergent total synthesis of the PP2A-inhibitor phoslactomycin A was achieved using a CuTC-mediated coupling of an alkenyl iodide C1-C13 fragment with an C14-C21 alkenyl stannane in the presence of a protected phosphate. Key features for the assembly of the C1-C13 fragment were an asymmetric dihydroxylation, an Evans-Aldol reaction, and a well-balanced protective group strategy. An asymmetri...

microRNAs (miRNAs/miRs) may have a crucial function in tumor metastasis through the regulation of a plethora of signaling pathways. Increasing evidence has shown that miR-199a is important in regulating the tumor metastasis of ovarian cancer, although the precise biological function of miR-199a is unclear at present. In the current study, it was observed that the expression levels of miR-199a w...

To probe the effect of the protein environment on the retinal chromophore of rhodopsin, we have performed molecular dynamics simulations using combined quantum mechanics / molecular mechanics (QM/MM). The starting geometry of the protein is based on the 2.6Å X-ray structure of bovine rhodopsin by Okada et al. The wild type chromophore of rhodopsin shows a highly twisted conformation around the ...

| Ensembles of multi-layer network is set up to predict the carbon-13 nuclear magnetic resonance (C13 NMR) chemical shifts of a series of mono-substituted benzenes. The descriptors (inputs) used are twelve structural-based vectors that correspond to the calculated H uckel and Gasteiger electron densities of the mono-substituted aromatic systems and four graphical descriptors that correspond to ...

We have examined whether active immunization with c13 protein, a hybrid protein of the first 81 amino acids of the viral NS1 nonstructural protein and the HA2 subunit of A/PR/8 (H1N1) hemagglutinin, could protect BALB/c mice from challenge with A/PR/8 H1 subtype virus. Mice immunized with the c13 protein had a significant reduction of pulmonary virus titers with A/PR/8 (H1) virus, but failed to...

El ácido elágico (AELG) es un metabolito en varias plantas y vegetales medicinales se puede encontrar libre o como parte de la molécula más compleja (elagitaninos). Esta investigación tuvo objetivo estudiar las interacciones AELG los aminoácidos (AA) tejidos humanos. software Hyperchem utilizó base para química cuántica. Se tomó teoría del coeficiente transferencia electrones (CTE). Los resulta...