A larger size of chromosomes in neoplastic cells than in cells of the corresponding normal tissues has been observed in a number of instances (2, 4, 6, 8, 9, 11, 13). Among the several proposed explanations, that of Biesele, Poyner, and Painter (9) suggested that the chromosomes of malignant cells contained muhiples of the number of chromosome strands present in those of normal cells. In order ...

C58 mice (aged greater than or equal to 5 months) are susceptible to age-dependent polioencephalomyelitis, a paralytic central nervous system disease induced by lactic dehydrogenase virus. Susceptibility results from the loss of protective T cells. Data are presented showing a positive correlation between the age-related loss of Lyt-1,2 cells and the development of susceptibility to neuroparaly...

Well-differentiated fibrosarcomas and anaplastic sarcomas developed in the s.c. tissues of four low-leukemia strains of mice (BALB/cf/Ki, Af/Ki, C3H/KÌand C3Hf/Ki) as well as two high-leukemia strains (AKR/Ki and C58/KÌ) following a single injection of benzpyrene (0.004 mg/g body weight) as young adults. The incidence of sarcomas was significantly less in both high-leukemia strains than that ...

Agrobacterium tumefaciens C58 grown on acidic medium containing glucose and solidified with bacteriological agar expressed a virB::lacZ fusion. No expression of this fusion was observed on a similar medium which was solidified with purified agarose. The fraction from bacteriological agar which was responsible for vir gene induction was extracted with methanol and partially purified by preparati...

Well-differentiated fibrosarcomas and anaplastic sarcomas developed in the s.c. tissues of four low-leukemia strains of mice (BALB/cf/Ki, Af/Ki, C3H/KÌand C3Hf/Ki) as well as two high-leukemia strains (AKR/Ki and C58/KÌ) following a single injection of benzpyrene (0.004 mg/g body weight) as young adults. The incidence of sarcomas was significantly less in both high-leukemia strains than that ...

We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and coo...

Motility of the protozoan parasite Toxoplasma gondii plays an important role in the parasite's life cycle and virulence within animal and human hosts. Motility is driven by a myosin motor complex that is highly conserved across the Phylum Apicomplexa. Two key components of this complex are the class XIV unconventional myosin, TgMyoA, and its associated light chain, TgMLC1. We previously showed ...

We have begun an investigation of the level of enzymatic activity in tissues which have been in filtrated by malignant cells. Two preliminary re ports of this work have appeared (1, @). We are presenting herewith results of studies of fatty acid oxidase activity which have been carried out on C58 mouse liver homogenates according to Lehn inger (3, 4). In addition, results of orienting ex perime...