Imidazole buffers were found to interfere with the determination of soluble proteins using Lowry's classical method. The influence of the constituent elements of the buffer on the calibration line was studied statistically. By combining the data corresponding to different experimental sequences, interserial calibration curves for different concentrations of imidazole buffer (10-30 mM) were obta...

The main objective of this research was to investigate the effect of material properties, strain-rate sensitivity, and barreling on the behavior of friction calibration curves. The compression tests were conducted to obtain the necessary material properties for the finite element analysis. A series of ring compression tests were then conducted in order to determine the magnitude of the friction...

Biological radiation dose can be estimated from dicentric chromosome frequencies in metaphase cells. Performing these cytogenetic dicentric chromosome assays is traditionally a manual, labor-intensive process not well suited to handle the volume of samples which may require examination in the wake of a mass casualty event. Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) soft...

OBJECTIVE Enzyme-linked immunosorbent assays (ELISAs) are widely used to quantify immunoglobulin levels induced by infection or vaccination. Compared to conventional manual assays, automated ELISA systems offer more accurate and reproducible results, faster turnaround times and cost effectiveness due to the use of multianalyte reagents. DESIGN The VaccZyme™ Human Anti-Haemophilus influenzae t...

Many prognostic systems have been devised to predict the outcome of liver transplantation (LT) candidates. Today, the Model for End-Stage Liver Disease (MELD) is widely used for organ allocation, but it has shown some limitations. The aim of this study was to investigate the performance of MELD compared to 5 different score models. We evaluated the prognostic ability of MELD, modified Child-Tur...

RATIONALE Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is used for the fast qualitative and quantitative analysis of phosphatidylcholines (PC). Fatty acyl chain lengths and the number of double bonds (DB) affect relative responses of PC; hence the determination of correction factors of individual PC is important for the accurate quantitation. The signal intensity in ...

This paper compares three methods of item calibration—concurrent calibration, separate calibration with linking, and fixed item parameter calibration—that are frequently used for linking item parameters to a base scale. Concurrent and separate calibrations were implemented using BILOG-MG. The Stocking and Lord (1983) characteristic curve method of parameter linking was used in conjunction with ...

In biosecure composting, animal mortalities are so completely isolated during the degradation process that visual inspection cannot be used to monitor progress or the process status. One novel approach is to monitor the volatile organic compounds (VOCs) released by decaying mortalities and to use them as biomarkers of the process status. A new method was developed to quantitatively analyze pote...

The objective of this study was to provide calibration curves for correcting intraocular pressure (IOP) measurements obtained using the Tono-Pen XL ® tonometer in cats, cows and sheep. Twelve eyes from 9 cats, 13 eyes from 7 cows, 10 eyes from 5 sheep were used. The anterior chamber of the eye was cannulated in vivo , in situ (immediately post mortem) or ex vivo with a fine needle and IOP was v...

We describe in this paper closed-form solutions to the following problems in multi-view geometry of n'th order curves: (i) recovery of the fundamental matrix from 4 or more conic matches in two views, (ii) recovery of the homography matrix from a single n'th order (n 3) matching curve and, in turn, recovery of the fundamental matrix from two matching n'th order planar curves, and (iii) 3D recon...