In this study, calli were obtained from in vitro grown potato stem or leaf segments cv. Cosima on MS medium containing 2,4-D, NAA, kinetin and yeast extract. After three subcultures, well grown calli were exposed to UV-C radiation with approximately 6.24 μmol photon/m2/s for 30 min three times at an interval of 2 weeks. Somaclonal variation induced by UV-C radiation was revealed by 5 out of 28 ...

We already reported that genetically engineered resveratrol-enriched rice (RR) showed to down-regulate skin melanogenesis. To be developed to increase the bioactivity of RR using calli from plants, RR was adopted for mass production using plant tissue culture technologies. In addition, high-pressure homogenization (HPH) was used to increase the biocompatibility and penetration of the calli from...

The study was conducted to standardize a protocol for Agrobacterium-mediated genetic transformation of buffel grass (Cenchrus ciliaris L.). Embryogenic calli, produced from one-year-old mature seeds of buffel grass, were used as target cells for Agrobacterium-mediated transformation. A. tumefaciens strain LBA4404, harbouring pCAMBIA-1301 or pCAMBIA-2301, was used for co-cultivation with embryog...

Transcription factors regulating the stress-responsive gene expression play an important role in plant stress adaptation. In this study, we examined the salt stress tolerance of transgenic Virginia pine (Pinus virginiana Mill.) overexpressing a Capsicum annuum ERF/AP2-type transcription factor (CaPF1), which may enhance the ability of transgenic plants to tolerate various kinds of stresses duri...

An efficient genetic transformation system for tall fescue ( Festuca arundinacea Schreb.), using Agrobacterium tumefaciens-mediated TDNA delivery, is described. Seed-derived embryogenic calli were infected with Agrobacterium tumefaciens strain EHA105 harboring plasmids pTOK47 and pCAMBIA1301. Infected calli were selected at 250 mg L 1 hyg B and the regenerated plantlets at 50 mg L . Using the p...

The physiological role of steroid hormones in humans is well known, and the metabolic pathway and mechanisms of action are almost completely elucidated. The role of plant steroid hormones, brassinosteroids, is less known, but an increasing amount of data on brassinosteroid biosynthesis is showing unexpected similarities between human and plant steroid metabolic pathways. Here we focus our atten...

The present study was aimed at the development of a somoclonal variant and isozyme marker for Phyllanthus amarus Schum & Thonn using inter-nodal segments and the enzyme peroxidase. Maximum callus proliferation was obtained on Murashige and Skoog’s medium supplemented (MS) with 1.0 mg/l of 2,4-Dichlorophenoxyacetic acid. Three weeks-old pale yellowish white semi-friable callus was used for organ...

The stability of aging barley calli was investigated with the barley retroelement 1 (BARE-1) retrotransposon specific inter-retrotransposon amplified polymorphism (IRAP) technique. Mature embryos of barley (Hordeum vulgare cv. Zafer-160) were cultured on callus induction MS medium supplemented with 3 mg/L 2,4-D and maintained on the same medium for 60 days. Ten IRAP primers were used in 25 diff...

High frequency somatic embryogenesis and plant regeneration was achieved on calli developed from internodal and flower buds of E. foetidum. The explants were cultured on Murashige and Skoog medium supplemented with different concentrations and combinations of BA and IAA. The nodal explants produced multiple adventitious shoots while the internodal and flower buds induced callus, from which mult...

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot indu...