In a previous publication (1) we have described an in vitro system for studying the callus growth on bean stem sections which is initiated by applying relatively high concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). Only short exposure to 2,4-D was necessary to initiate the callus growth. Since this growth is not tumorous in nature and therefore not auxin autonomous, it will continue o...

The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with various growth hormones like NAA, IAA, IBA and 2, 4-D individually and in various combinations were checked for callus induction. Among the growth...

Introduction Cumin (Cuminum cyminum L.) is a commercial plant which is valued for its aroma and its medicinal and therapeutic properties. Although India has been the primary exporter of cumin seeds and cumin oil in the world. However Turkey and Iran are providing stiff competition now. India, Turkey, Syria, China, the US, Iran, Indonesia, Sudan, Egypt, Morocco, Algeria, and Libya are the leadin...

An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested...

A methanol extract of Gymnema sylvestre leaf and callus showed anti-diabetic activities through regenerating β-cells. Optimum callus was developed under stress conditions of blue light with 2,4-D (1.5 mg/l) and KN (0.5 mg/l), which induced maximum biomass of green compact callus at 45 days, as determined by growth curve analysis. Leaf and optimum callus extracts contains gymnemic acid, which wa...

Plants develop unorganized cell masses like callus and tumors in response to various biotic and abiotic stimuli. Since the historical discovery that the combination of two growth-promoting hormones, auxin and cytokinin, induces callus from plant explants in vitro, this experimental system has been used extensively in both basic research and horticultural applications. The molecular basis of cal...

leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...

Vitamins are necessary compounds synthesized and utilized in plants. In tissue culture media, vitamin addition is not always common; since the amount needed by plants is relatively unknown and varies. Vitamins, in combination with other media constituents, have been shown to have direct and indirect effects on callus growth, somatic growth, rooting, and embryonic development. For example, diffe...

The effects of eight different narrow band-emitting fluorescent lamps (371-750 nm) and four commercial broad band-emitting fluorescent sources upon growth and shoot initiation in tobacco callus (Nicotiana tabacum var. Wisconsin 38) have been characterized. Wavelength and intensity are equally important parameters in determining morphogenic changes. Near ultraviolet light (371 nm) was found to s...

The production of kencur (Kaempferia galanga L.) with tissue culture is an alternative in plant cultivation as a provider seeds. In culture, growth regulators can affect the formation organs (organogenesis). combination NAA and TDZ was used this study to induce callus organogenesis. purpose research determine effect giving on organogenesis callus, well optimal two callus. design factorial RAL f...