We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in Escherichia col...

1. SUMMARY Campylobacter jejuni and related species are important human pathogens, causing acute human enterocolitis, and they are the most common cause of food-borne diarrhoea in many industrialized countries. Previous infection with certain strains of C. jejuni is also linked with the development of the neurological disorder Guillain±Barre  syndrome (GBS). Despite its importance as a human p...

We designed polymearse chain reaction (PCR) primers to distinguish Campylobacter jejuni from Campylobacter coli and to differentiate Arcobacter from other species of Campylobacter. We applied these PCR methods to estimate their prevalence in feces of healthy cattle and hogs and to identify risk factors for infection. For cattle, C. jejuni (23%), Arcobacter (11%), and C. coli (1.57%) were detect...

The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. These species may be found in water, food and in the intestinal tract of chickens. This study investigated the presence of Campylobacter jejuni and Campylobacter coli in chicken abattoirs in São Paulo State, Brazil. A total of 288 samples of feces, feathers, scald water, ...

The study was designed with a view to isolate, identifies and characterizes Campylobacter species from broiler meat samples (leg muscle, breast muscle and cloacal skin) which were collected from KR market at Bangladesh Agricultural University, Mymensingh during the period from January 2013 to May 2013. A total of 50 samples were subjected to bacterial isolation and identification by using cultu...

Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolat...

The main aim of this study was to combine the techniques of most probable number (MPN) and polymerase chain reaction (PCR) for quantifying the prevalence and numbers of Campylobacter spp. in ulam, a popular Malaysian salad dish, from a traditional wet market and two modern supermarkets in Selangor, Malaysia. A total of 309 samples of raw vegetables which are used in ulam were examined in the st...

Rectal swabs and stool specimens were compared for the detection of Campylobacter fetus subsp. jejuni in marmosets. Rectal swabs were superior to stool specimens for detection of Campylobacter fetus subsp. jejuni (P = 0.016). Preliminary human data are also presented.

Two cases of acute enteritis due to Campylobacter jejuni infection are described. The Authors remark the clinical variability this not so rare and discuss indication antibiotic treatment.

The prevalence of Campylobacter jejuni and Campylobacter coli and their biotypes in beef and dairy cattle from the South of Chile was established. Campylobacter were statistically more prevalent among beef cattle (35.9%) than among dairy cattle (21.3%), being C. jejuni the species most frequently isolated.