Microtubules (MTs) polymerize via net addition of GTP-tubulin subunits to the MT plus end, which subsequently hydrolyze to GDP-tubulin in the MT lattice. Relatively stable GTP-tubulin subunits create a "GTP cap" at the growing MT plus end that suppresses catastrophe. To understand MT assembly regulation, we need to understand GTP hydrolysis reaction kinetics and the GTP cap size. In vitro, the ...

Purpose: With the recent discovery that -methlyacly-coenzyme A racemase (AMACR) is over expressed in a majority of prostate cancer (CaP) specimens we developed a novel polymerase chain reaction (PCR) based approach that would predict the presence of CaP from prostatic secretions. Materials and Methods: A total of 21 patients were enlisted in this study, including 10 with CaP, 2 with high grade ...

Background: Carcinoma cervix is the second most common cancer in women (18%). Cervical smear by routine Papanicolaou (Pap) screening has significant rates of false-positive and false-negative results. To minimise this, we compare efficacy cervical acid phosphatase-papanicolaou (CAP-Pap) with conventional Pap detecting premalignant lesions cervix. The red-colored granules (enzyme coated abnormal...

objective(s): the aim of the present study is to determine the effect of chloramphenicol (cap) on rat liver and small intestine. effect of phenobarbital (pb) on cap toxicity was also investigated.   materials and methods:   rats were received cap at doses of 0, 200, 400 and 600 mg/kg. another group was pretreated with 80 mg/kg pb 30 min prior to administration of various doses of cap. the exper...

The influence of electrical stimulation on the amplitude of the action potentials recorded from the mouse nervous system in vitro was investigated. Brief (1 s) high frequency (100 Hz) stimulation of the sciatic nerve induced a longlasting increase in the amplitude of the compound action potential (CAP). Low frequency (1 Hz) stimulation delivered for 15 min attenuated the antidromically evoked p...