Polypyrrole (PPy) was chemically synthesised at two pH levels (pH = 2 and unadjusted pH, i.e. 6.6) using pre-formed carboxymethylcelluloseiron (CMC-Fe) complexes. The CMC-Fe complexes were prepared at a fixed CMC concentration, i.e. 5.5x10 mol/L, and with an increasing FeCl3 amount (from 4x10 to 5x10 mol/L). The quantity of iron bound to CMC was determined by the inductively coupled plasma (ICP...

A gene encoding a glycoside hydrolase family 44 (GH44) protein from Clostridium acetobutylicum ATCC 824 was synthesized and transformed into Escherichia coli. The previously uncharacterized protein was expressed with a C-terminal His tag and purified by nickel-nitrilotriacetic acid affinity chromatography. Crystallization and X-ray diffraction to a 2.2-A resolution revealed a triose phosphate i...

One hour after the injection of 5 fie. of L-lysine-U-C14 into each of a group of rats bearing the Walker 256 carcinosarcoma, the acid-soluble proteins were extracted from nuclear preparations of the tumor. The proteins of these extracts were chromatographed on carboxymethylcellulose, with formic acid as the eluting agent. Rechromatography of 150 mg. of RP2-L1 on carboxymethylcellulose resulted ...

The effect of the interaction of iota-carrageenan (ICG) or lambda-carrageenan (LCG) with locust bean gum and carboxymethylcellulose on the properties of ice cream was studied employing a mixture design approach. A model formulation was employed with of 5% (w / w) of hydrocolloids like stabilizers. Hydrocolloids mixture effect on ice cream properties (base viscosity, ice-cream overrun, hardness,...

EVSTATIEVA, Y., M. BADALOVA, G. CHERNEV, D. NIKOLOVA and V. SAVOV, 2013. New hybrid matrix for immobilization of Trichoderma viride SL-45. Bulg. J. Agric. Sci., Supplement 2, 19: 120–122 In this work Trichoderma viride SL-45, was investigated in new a hybrid matrix by immobilizing method. Vegetative culture of this strain was immobilized in four variations of sol-gel hybrid silica materials, co...

PURPOSE Artificial tear formulations typically contain a water-soluble polymer to enhance residence time, moisture retention, and binding to the mucin coat of the ocular surface, which facilitate corneal healing. This study investigated the potential advantages of combining carboxymethylcellulose (CMC) and hyaluronic acid (HA) polymers in a single formulation. MATERIALS AND METHODS Individual...

The proteins of the large subunit of rat liver ribosomes were separated into seven groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5. Seventeen proteins (L4, L5, Li’, L9, Lll, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35’, L37, and L39) were isolated from three of the groups (B60, D60, G60) by ion exchange chromatography on carboxymethylcellulose and by filtration t...