Actin-based organelle movements are driven by the related multifunctional myosin motors of class V in animals and fungi and class XI in plants. The versatility of these motors depends critically on their C-terminal globular tail domain that allows them to bind to a broad variety of cargo molecules. Regulation of this motor-cargo attachment is frequently employed to modulate organelle movement. ...

The exomer complex is a putative vesicle coat required for the direct transport of a subset of cargoes from the trans-Golgi network (TGN) to the plasma membrane. Exomer comprises Chs5p and the ChAPs family of proteins (Chs6p, Bud7p, Bch1p, and Bch2p), which are believed to act as cargo receptors. In particular, Chs6p is required for the transport of the chitin synthase Chs3p to the bud neck. Ho...

The AP2 adaptor complex (alpha, beta2, sigma2, and mu2 subunits) crosslinks the endocytic clathrin scaffold to PtdIns4,5P(2)-containing membranes and transmembrane protein cargo. In the "locked" cytosolic form, AP2's binding sites for the two endocytic motifs, YxxPhi on the C-terminal domain of mu2 (C-mu2) and [ED]xxxL[LI] on sigma2, are blocked by parts of beta2. Using protein crystallography,...

Purpose: To determine resource rational allocation in the management of technique that specializes intra-terminal moving, to develop dispatching method for container flow. Methods: The work applies materials from open sources. Systematic approach is applied designing system terminal technique. Results: Method flow at has been developed, features combining conversion processes on basis prioritiz...

background: anecdotal information has suggested that sodium benzoate is used with more than permissible doses during production steps of food products especially pickles and pickled cucumbers in food producing factories in markazi province and other food producing factories . the present study was done to evaluate factual concentration of sodium benzoate in these products. methods: in this stud...

The Sar1 GTPase is an essential component of COPII vesicle coats involved in export of cargo from the ER. We report the 1.7-A structure of Sar1 and find that consistent with the sequence divergence of Sar1 from Arf family GTPases, Sar1 is structurally distinct. In particular, we show that the Sar1 NH2 terminus contains two regions: an NH2-terminal extension containing an evolutionary conserved ...

Internalization of diverse transmembrane cargos from the plasma membrane requires a similarly diverse array of specialized adaptors, yet only a few adaptors have been characterized. We report the identification of the muniscin family of endocytic adaptors that is conserved from yeast to human beings. Solving the structures of yeast muniscin domains confirmed the unique combination of an N-termi...

ABSTRACT In animal cells, a single cytoplasmic dynein motor mediates microtubule minus-end-directed transport, counterbalancing dozens of plus-end-directed kinesins. The remarkable ability to interact with diverse cargo spectrum stems from its tightly regulated recruitment cargo-specific adaptor proteins, which engage the dynactin complex make tripartite processive motor. Adaptor binding is gov...

An international study of the airport choice factors for non-integrated cargo airlines By John Gardiner Recent forecasts predict that the current number of all-cargo aircraft worldwide will more than double over the next 20 years, spurred on by trends such as reduced passenger belly hold capacity on short and medium haul routes and a growing recognition of the profit potential of cargo by airli...