Bacterial enzymes have long been considered solely accountable for the degradation of the dentin matrix during the carious process. However, the emerging literature suggests that host-derived enzymes, and in particular the matrix metalloproteinases (MMPs) contained in dentin and saliva can play a major role in this process by their ability to degrade the dentin matrix from within. These finding...

This study was designed to identify surface and subsurface microscopic changes in different carious lesions by using Confocal Laser Scanning Microscope (CLSM) and Image analyzer (light microscopy). Thirty extracted carious posterior teeth were fixed, embedded and polymerized in plastic fixation medium. The final thin sections (80mm) were stained with H&E and Masson Goldner's Tricome while other...

MMP-20 (enamelysin), the matrix metalloproteinase family member discovered in the enamel organ, has also been detected in odontoblasts during dentin formation. We studied the presence and localization of MMP-20 in mature human teeth in health and disease. In immunohistochemistry, MMP-20-positive staining was observed most intensively in the radicular odontoblastic layer and also in dilated dent...

BACKGROUND Currently, several systems of dentin substrate-reacting adhesives are available for use in the restorative treatment against caries. However, the bond effectiveness and property of different adhesive systems to caries-affected dentin are not fully understood. The objective of this study was to evaluate the bond strength of different adhesives to both normal dentin (ND) and caries-aff...

objective: the aim of this study was to evaluate the microtensile bond strength to sound and caries-affected dentin using single bond and adper prompt-l-pop adhesives. materials and methods: sixteen extracted human molars with carious lesions extended halfway through dentin were ground to expose the caries affected and the surrounding normal dentin. the samples were divided into two groups of e...

The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Sev...

Aim: To evaluate the comparative efficacy of Polymer bur (Smartprep) and Conventional burs (Carbide bur & Diamond Points) in selective Dentin caries removal. Materials and method: 15 freshly extracted carious human premolars and molars were selected for this study. These teeth were split in the center of carious lesion, in order to obtain two corresponding half. Thus 30 samples were prepared in...

Variations of calcium45 taken up in vitro by the enamel and dentin of teeth have been reported recently. Dentin appears to adsorb more Ca45 than enamel, and carious dentin more than non-carious dentin. Underwood and Hodge'0 measured the Ca45 differences in solutions of powdered non-carious enamel and dentin. Minder and Gordonoff7 have published auto-radiographs with Ca45 of intact rat teeth, an...