Carpesium abrotanoides L. is rich in sesquiterpene lactones, the experiments of anti-tumor activity vitro showed that total lactone extracts exhibited most cytotoxicity activities against HepG-2 cells (IC50 = 4.2 μg/mL). In order to isolated active compounds quickly, strategy HPLC-ESI-Q-TOF-MS/MS combined with high-speed counter-current chromatography (HSCCC) was implemented. Three compounds, 2...