Casein kinase II from bovine brain transfers about one mole of phosphate to a serine residue near the COOH terminus of the heavy chain of myosin isolated from bovine brain. We have purified and characterized a peptide that contains this phosphoserine. The peptide was generated by chymotryptic and thermolytic digestion and was isolated by gel filtration, Fe3+ affinity chromatography, and reverse...

Here we report the molecular cloning and sequencing of three types of human a81-casein transcripts and present evidence indicating that exon skipping is responsible for deleted mRNA transcripts. The largest transcript comprised 981 bp encoding a signal peptide of 15 amino acids followed by the mature a.1casein sequence of 170 amino acids. Human asl-casein has been reported to exist naturally as...

background and objectives: proteases are a group of enzymes that catalyze the degradation of proteins resulting in the pro- duction of their amino acid constituents. they are the most important group of industrial enzymes which account for about 60% of total enzymes in the market and produced mainly by microorganisms. the attempts were made to study the kinetic parameters of protease produced b...

Phosphorylation of rabbit muscle glycogen synthase by cyclic AMP-dependent protein kinase has been shown to enhance subsequent phosphorylation by casein kinase I (Flotow, H., and Roach, P. J. (1989) J. Biol. Chem. 264, 9126-9128). In the present study, synthetic peptides based on the sequences of the four phosphorylated regions in muscle glycogen synthase were used to probe the role of substrat...

Production of iron-chelating peptides from protein hydrolysates requires robust and adequate screening methods to optimize their purification subsequently valorize potential antioxidant properties. An original methodology was developed for direct sensitive iron(II)-chelating based on ion-pair reverse phase liquid chromatography (IP-RPLC) coupled high-resolution mass spectrometry (HRMS). Peptide...

Abstract Food components possessing zinc ligands can be used to inhibit zinc-dependent enzymes. In this study, zinc-binding peptides were derived from whey protein hydrolysates, and their ultrafiltration (> 1 < kDa) fractions, produced with Esperase (WPH-Esp), Everlase Savinase. Immobilized metal affinity chromatography (IMAC-Zn 2+ ) increased the capacity of peptide fraction (83%) when c...

Human erythrocyte band 3 is a major substrate of two red blood cell protein kinases, casein kinase I and p72syk protein tyrosine kinase. Although the phosphorylation sites and physiologic consequences of p72syk phosphorylation have been characterized, little is known regarding casein kinase I phosphorylation. In this report, we identify the major phosphorylation site of casein kinase I. Using i...

In adults, dietary protein seems to induce weight loss and dairy proteins may be insulinotropic. However, the effect of milk proteins in adolescents is unclear. The objective was to test whether milk and milk proteins reduce body weight, waist circumference, homeostatic model assessment, plasma insulin, and insulin secretion estimated as the plasma C-peptide concentration in overweight adolesce...

The SH2 and SH3 domain structural integrity and functionality were confirmed by peptide binding assays. Binding studies using two phosphotyrosine peptides derived from EGFR (FLPVPE{pY}IN-QSVPKR) and Shc (EEPPDHQ{pY}YNDFPGK) for the Grb2 SH2 domain and a SOS1-derived proline-rich peptide (PVPPPVPPRRRPEY) for the SH3 domains were performed using MST 1. Grb2 was fluorescently labeled using Atto488...

The antioxidant capacity of porcine splenic hydrolysate (PSH) was studied in vitro and in vivo. Peptide hydrolysates were prepared, using the proteolytic enzyme Alcalase(®). The molecular weights of PSH were 37,666, 10,673, 6,029, and 2,918 g/mol. Rats were fed a 5% (w/v) PSH diet, instead of a casein diet, for 4 wk. The food intake, body weight gain, and liver weight of rats in the PSH group w...