introduction: human hematopoietic stem cells (hhscs) have been used for transplantation in hematologic failures. because the number of hhscs per cord blood unit is limited, the expansion of these cells is important for clinical application. it has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hhscs. in this regard, cord blood cd34+ cells expanded a...

Introduction: Human hematopoietic stem cells (hHSCs) have been used for transplantation in hematologic failures. Because the number of hHSCs per cord blood unit is limited, the expansion of these cells is important for clinical application. It has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hHSCs. In this regard, cord blood CD34+ cells ex...

introduction: umbilical cord blood (cb) has been identified as a rich source for hematopoietic stem cells (hscs), and has provided an alternative to bone marrow transplantation. the use of ex vivo expanded cells has been suggested as a possible means to accelerate the speed of engraftment in cord blood (cb) transplantation. the main aim of our study is to find the best culture media and conditi...

introduction: while human endothelial progenitor cells (epcs) have been a subject of somehow extensive investigation, epcs from adult mouse hematopoietic system were poorly studied. present investigation is focused on fvb mouse endothelial progenitor cells in terms of their isolation, purification, and expansion. materials and methods: mononuclear cells collected from murine peripheral blood we...

Here a new, intrinsically pluripotent, CD45-negative population from human cord blood, termed unrestricted somatic stem cells (USSCs) is described. This rare population grows adherently and can be expanded to 10(15) cells without losing pluripotency. In vitro USSCs showed homogeneous differentiation into osteoblasts, chondroblasts, adipocytes, and hematopoietic and neural cells including astroc...

Abstract Background B cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. It has been shown that B cells produced successfully in the presence of stem cell factor (SCF) and Flt3 ligand (Flt3L). IL2, IL7, and IL15 cytokines as -common family have an essential cytokines may support either synergistically or in an additive m...

cd34 is a type i membrane protein with a molecular mass of approximately 110 kda. this antigen is associated with human hematopoietic progenitor cells and is a differentiation stage-specific leukocyte antigen. in this study we have generated and characterized monoclonal antibodies (mabs) directed against a cd34 marker. mice were immunized with two keyhole lympet hemocyanin (klh)-conjugated cd34...

Furosemid (FUR) adalah obat diuretik kuat yang umum dipakai untuk pengobatan tekanan darah tinggi dan lemah jantung. Bahan baku FUR memiliki kemampuan mengalir (flowability) dikempa menjadi tablet (tabletability) buruk. Pada penelitian memperbaiki flowability tabletability furosemid diperbaiki melalui pembentukkan ko-kristal dengan kafein (CAF). Metode Ultrasound Assisted Solution Co-Crystaliza...

Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...