Objective : To define the best combination of biomarkers for diagnosis infection and sepsis in emergency room. Methods In this prospective study, consecutive patients with a suspicion room were included. Eighteen different measured plasma, twelve on monocytes, neutrophils, B T-lymphocytes studied combinations determined by gradient tree boosting approach. Results Overall, 291 included analysed,...

Abstract Background The abundance of human milk leukocytes, including neutrophils, changes in response to the health status mothers and their infants. Elevated leukocyte counts occur during infant infection return baseline levels upon recovery. However, it is unclear how can affect neutrophil phenotypes concentration fatty acids mothers’ milk. Objectives This study investigates association betw...

Results: We found high percentage of expression of CD64 on neutrophils in patients (67.73 ± 24.74) when compared with controls (8.19 ± 3.64) and also their percentage of expression was higher in culture positive sepsis (76.25 ± 29.07) than culture negative sepsis (42.21 ± 33.24). In the present study, I/T ratio >0.2 had a sensitivity, specificity, PPV, NPV and accuracy i.e. 70.0%, 72.0%, 33.3%,...

Current systems for conditional gene deletion within mouse macrophage lineages are limited by ectopic activity or low efficiency. In this study, we generated a Mafb-driven Cre strain to determine whether any dendritic cells (DCs) identified by Zbtb46-GFP expression originate from a Mafb-expressing population. Lineage tracing distinguished macrophages from classical DCs, neutrophils, and B cells...

Effector dysfunctions of neutrophil granulocytes are often associated with the occurrence dysregulatory processes in antibacterial immune defense. Acute destructive pneumonia is a severe purulent-inflammatory disease discordant functions effector mechanisms and emergence negatively transformed cell subsets. Therefore, search for new experimental approaches aimed at re-orientation altered phenot...

The distinction between causes of acute infections is a major clinical challenge. Current biomarkers, however, are not sufficiently accurate. Human neutrophil lipocalin (HNL) concentrations in serum or whole blood activated by formyl-methionine-leucine-phenylalanine (fMLP) were shown to distinguish acute infections of bacterial or viral cause with high accuracy. The aim was therefore to compare...

BACKGROUND AND PURPOSE Polymorphonuclear neutrophils (PMNs) act as a first line of defense against bacterial infections. Recent studies indicated that PMNs could act as antigen-presenting cells. The aim of this study was to detect the expression of major histocompatibility complex (MHC) class II and certain molecules and receptors (CD83, CD64, and CD14) on the surface of PMNs stimulated with in...

BACKGROUND Cat allergen Fel d 1 is a heterodimer encoded by 2 separate genes that has been difficult to produce as a fully immunoreactive molecule. OBJECTIVE We sought to engineer recombinant (r) Fel d 1 with IgE and IgG antibody binding comparable with that of the natural allergen that could be targeted to antigen-presenting cells. METHODS The rFel d 1 chains were coexpressed in baculoviru...