Particle counting in microfluidic devices with coulter principle finds many applications in health and medicine. Cell enumeration using microfluidic particle counters is fast and requires small volumes of sample, and is being used for disease diagnostics in humans and animals. A complete characterization of the cell counting process is critical for accurate cell counting especially in complex s...

Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. The manual method can h...

Stereologic cell counting has had a major impact on the field of neuroscience. A major bottleneck in stereologic cell counting is that the user must manually decide whether or not each cell is counted according to three-dimensional (3D) stereologic counting rules by visual inspection within hundreds of microscopic fields-of-view per investigated brain or brain region. Reliance on visual inspect...

Overlapping white blood cell identification on microscopic blood cell images is proposed for increasing the accuracy of white blood cell segmentation and counting. The accurate identification of overlapping cells can increase the accuracy of cell counting system for diagnosing diseases. The overlapping cells have different characteristic such as area and shape with a single cell of microscopic ...

To address the scale variance and uneven distribution of objects in scenarios object-counting tasks, an algorithm called Refinement Network (RefNet) is exploited. The proposed top-down scheme sequentially aggregates multiscale features, which are laterally connected with low-level information. Trained by a multiresolution density regression loss, set intermediate-density maps estimated on each ...