Yeast immobilization with low-cost carrier materials is a suitable strategy to optimize the fermentation of lignocellulosic hydrolysates for production second-generation (2G) ethanol. It defined as physical confinement intact cells certain region space (the carrier) preservation their biological activity. This technological approach facilitates promising strategies bioethanol due enhancement pe...

In whole-cell based biosensors, spectrophotometry is one of the most commonly used methods for detecting organophosphates due to its simplicity and reliability. The sensor performance is directly affected by the cell immobilization method because it determines the amount of cells, the mass transfer rate, and the stability. In this study, we demonstrated that our previously-reported microbe immo...

BACKGROUND Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enzymatic activity, and permits repeated or continuous biocatalyst use. However, conventional immobilization methods have practical limitations, such as limited mass transfer in th...

Bacterial cellulose (BC) was used as a modern carrier in immobilization of cell. The immobilization of Corynebacterium glutamicum using bacterial cellulose (BC) as a carrier was carried out in two steps: adsorption and incubation. The screening method Plackett-Burman Design was used to identify the significantly influenced factors and the experiments were designed by Response Surface Methodolog...

Nanoporous gold (referred to as np-Au or NPG) has emerged over the past 10 years as a new support for enzyme immobilization. The material has appealing features of ease of preparation, tunability of pore size, high surface to volume ratio, and compatibility with multiple strategies for enzyme immobilization. The np-Au material is especially of interest for immobilization of redox enzymes for bi...

Despite their sustainability, the potential of biocatalytic processes in industrial production is far from being realized. The main challenges this field are development highly active, robust, and stable biocatalysts, efficient regeneration cofactors, prevention biocatalyst deactivation under harsh conditions. In addition to engineering, enzyme cell immobilization plays a crucial role process f...

Anti-CD3 (aCD3) nanoarrays fabricated by self-assembled nanopatterning combined with site-directed protein immobilization techniques represent a novel T cell stimulatory platform that allows tight control over ligand orientation and surface density. Here, we show that activation of primary human CD4+ T cells, defined by CD69 upregulation, IL-2 production and cell proliferation, correlates with ...

The method to modify the surface of polystyrene (PS) substrate for stable and versatile biomolecule immobilization was developed, which consists of two steps: 1) surface treatment by argon plasma irradiation to introduce hydroxyl group on PS and 2) covalent immobilization of vinylmethyleter-maleic acid copolymer (VEMAC) through a coupling reaction between hydroxyl group on plasma-irradiated PS ...

We developed selective transfection of single particle sensor into a specific cell by optically controlled cell immobilization and membrane fusion of liposome for intracellular measurement. The particle was encapsulated in the liposome using packaging based on multi-layering method. The liposomes contain 1,3,3-Trimethylindolino-6'nitrobenzopyrylospiran (SP) that controls the surface potential o...