We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation...

Liquid methanol and methanol clusters have been comprehensively studied to reveal their local structure and hydrogen bond networks. However, our understanding of the crystal forms of methanol is rather limited. The known crystal structures of solid methanol, α, β, and γ, are composed of infinite hydrogen bond chains in their unit cell. The structural diversity of solid methanol is much less tha...

A near-infrared fluorescent probe (Cy-O-CHO) for the detection of endogenous Cys/Hcy in living cells was designed and synthesized. Cy-O-CHO exhibited high sensitivity and good selectivity to Cys/Hcy under physiological conditions with a detection limit of 7.9 nM for Cys.

In the present paper, we obtain some interesting properties for mero-morphic analytic functions related to Cho-Kwon-Srivastava Operator defined by means of an operator recently given by Ghanim and Darus.

We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins.

The cytotoxic action of 5-(3,3-dimethyll-triazeno)imidazole-4-carboxamide in Chinese hamster ovary cells is enhanced considerably in the presence oflight, when 5-diazoimidazole-4-carboxamide is being continu ously generated. A broad shoulder threshold-type survival curve is seen when the cells are exposed to 5-(3,3-dimethyll-triazeno)imidazole-4-carboxamide (both in light anddark). The drug had...

The aim of this paper is to present a systematic methodology to design macroscopic bioreaction models for cell cultures based upon metabolic networks. The cell culture is seen as a succession of phases. During each phase, a metabolic network represents the set of reactions occurring in the cell. Then, through the use of the elementary flux modes, these metabolic networks are used to derive macr...

A 2-year-old multiparous (Case 1) and a 2.5-year-old nulliparous 2) Djungarian hamster each presented with history of prolapsed mass from the vulva. partial uterine prolapse was diagnosed in both cases, according to clinical diagnostic examinations. The replaced hamster, an ovariohysterectomy performed. histopathological examination removed tissues revealed cyst papillary hyperplasia ovary. Thi...

The human O6-alkylguanine-DNA alkyltransferase complementary DNA was transfected into the alkyltransferase-deficient Chinese hamster ovary cell line, D422, in an effort to dissect the underlying mechanisms of bischloroethylnitrosourea (BCNU)-induced mutations. The alkyltransferase-transformed cell line exhibited 100-fold protection against BCNU-induced toxicity and an overall decrease in mutati...