Linolenate has been shown [I] to inhibit photosynthesis in intact spinach chloroplasts by inhibiting the influx of orthophosphate and the efflux of 3-phosphoglyceraldehyde. The inhibitory effect of linolenate was shown to depend on the level of the internal pool of metabolites [2] as a result of experiments which on the one hand used chloroplasts from preilluminated leaves or chloroplasts which...

Plastids are multifunctional, pleomorphic organelles of purported endosymbiotic origin that in plants and green algae display a characteristic double membrane envelope (Wise, 2007). All plastids originate fromcolorless proplastids, and a simple pigmentation-based classification distinguishes chloroplasts from other plastids by the presence of chlorophyll, chromoplasts by the predominance of oth...

Plastids are multifunctional, pleomorphic organelles of purported endosymbiotic origin that in plants and green algae display a characteristic double membrane envelope (Wise, 2007). All plastids originate fromcolorless proplastids, and a simple pigmentation-based classification distinguishes chloroplasts from other plastids by the presence of chlorophyll, chromoplasts by the predominance of oth...

(14)CO(2) photoassimilation in the presence of MgATP, MgADP, and MgAMP was investigated using intact chloroplasts from Sedum praealtum, a Crassulacean acid metabolism plant, and two C(3) plants: spinach and peas. Inasmuch as free ATP, ADP, AMP, and uncomplexed Mg(2+) were present in the assays, their influence upon CO(2) assimilation was also examined. Free Mg(2+) was inhibitory with all chloro...

In this experiment, the action mechanism of gene Ssc1 was studied in process interaction with plants through heterologous expression, subcellular localization and fluorescent PCR technology. It found that could enhance pathogenicity Botrytis cinerea expression. Fusing promoter, SP CTP GFP expressing tobacco, it fusion protein be secreted into plant cells located chloroplasts. Trypan blue staini...

The bacterial Sec and signal recognition particle (ffh-dependent) protein translocation mechanisms are conserved between prokaryotes and higher plant chloroplasts. A third translocation mechanism in chloroplasts [the proton concentration difference (DeltapH) pathway] was previously thought to be unique. The hcf106 mutation of maize disrupts the localization of proteins transported through this ...

Dynamin-like proteins (DLPs) are a family of membrane-active proteins with low sequence identity. The proteins operate in different organelles in eukaryotic cells, where they trigger vesicle formation, membrane fusion, or organelle division. As discussed here, representatives of this protein family have also been identified in chloroplasts and DLPs are very common in cyanobacteria. Since cyanob...

Protons, AsO;, and NH: are competitive inhibitors of Pi in the oxidative esterification of Pi in chloroplasts. Pi, AsO;, NH:, and other monovalent cations are competitive inhibitors of H+ in the cation-H+ exchange reaction of chloroplasts. Since Pi and protons compete for the same electron-activated fixed anionic sites in chloroplasts, it is proposed that the initial energy-requiring step in ox...

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

A monospecific antiserum to tobacco plastocyanin agglutinates stroma-free swellable chloroplasts from wild type tobacco, (Nicotiana tobacum var. John William's Broadleaf) from the tobacco aurea mutant Su/su2, (Nicotiana tabacum var. Su/su2) from Antirrhinum majus and spinach (Spinacia oleracea). In this condition the antiserum inhibits linear photosynthetic electron flow in tobacco and spinach ...