Isometric tension changes of the bovine iris dilator muscle were investigated in vitro. Electrical field stimulation (0.03-1 msec duration) produced a relaxation, which was abolished by the addition of tetrodotoxin, thereby suggesting a neuronal origin. Marked relaxation was also initiated in high K solution. Both of these relaxations were potentiated by neostigmine, while atropine abolished th...

Acetylcholinesterase has important role in synaptic cleft. It breaks down the acetylcholineatcholinergic synapsesand terminates the cholinergic effects. Some chemical agents likeorganophosphorus compounds (OPCs) including nerve agents and pesticides react withacetylcholinesteraseirreversibly. They inhibit normal biological enzyme action and resultin accumulation of acetylcholineand show toxic e...

Cholinergic agonists have previously been shown to augment the ability of sensitized lymphocytes to injure cells bearing the sensitizing alleantigens. The cholinergic receptor of the attacking lymphocyte population has been studied with pharmacological manipulation of an in vitro system that quantitates the injury mediated by sensitized attacking cells upon target cells. The data reveal that mu...

Acetylcholinesterase has important role in synaptic cleft. It breaks down the acetylcholineatcholinergic synapsesand terminates the cholinergic effects. Some chemical agents likeorganophosphorus compounds (OPCs) including nerve agents and pesticides react withacetylcholinesteraseirreversibly. They inhibit normal biological enzyme action and resultin accumulation of acetylcholineand show toxic e...

Organophosphate (OP) poisoning is one of the most common toxicological emergencies, especially in developing countries. They work by inhibiting cholinesterase enzyme lead to severe cholinergic toxicities. Organophosphorus intoxication occurs after cutaneous exposure, respiratory inhalation or gastrointestinal tract absorption. Exposure to organophosphate and other agents increases yearly, with ...

“Light” nerve endings from guinea pig cerebral cortex were incubated with [32P]orthophosphate, myo[3H]inositol, [3H]glycerol, or [3H]glucose in the presence or absence of cholinergic agents. Acetylcholine and carbamylcholine increased incorporation of “Pi into phosphatidic acid 100% and into various classes of phosphatidylinositol 20 to 70%. These agents also stimulated the labeling of phosphat...