Introduction: Chondrogenesis occurring in soft callus is one of the major histological processes in the healing of fractures, though the molecular and cellular events, which regulate this process, have not been fully elucidated. In the present study, using a standardized rat closed fracture model, we examined the spatial and temporal gene expression for cartilage-related proteins and extensivel...

This study is a continuing investigation of the effect of the brachypod mouse mutation on cell interactions and chondrogenesis during early limb development. In this report, cell adhesiveness was assessed in fused fragments of brachypod and normal limb-bud mesenchyme. Examination of the interface of fused distal postaxial limb fragments show brachypod limb mesenchyme to be more adhesive than no...

Cartilage has limited repair and regeneration capacity, thus damage of cartilage often results in its dysfunction and even chronic diseases like osteoarthritis (OA). Chondrogenesis induced by tissue-engineering methods is essential to treating cartilage-related diseases. MicroRNAs (miRNAs) are a class of small non-coding single-stranded RNAs which exert their biological effects by binding to th...

PURPOSE The purpose of this study was to evaluate the metabolomic changes in 3D-cultured human mesenchymal stem cells (hMSCs) in alginate beads, so as to identify biomarkers during chondrogenesis using (1)H nuclear magnetic resonance (NMR) spectroscopy. MATERIALS AND METHODS hMSCs (2×10(6) cells/mL) were seeded into alginate beads, and chondrogenesis was allowed to progress for 15 days. NMR s...

Chondrogenesis; chondromodulin-1 (chm1); Angiogenesis inhibitor; Cartilage; Notochord; Zebra®sh; type II collagen (col2a1); Craniofacial; Otic vesicle; Pectoral ®n; Pharyngeal arch; Maternal transcript; Trabecula; Ethmoid plate; Genetic locus 105 157 chondromodulin-1 (chm1); Angiogenesis inhibitor; Cartilage; Notochord; Zebra®sh; type II collagen (col2a1); Craniofacial; Chondrogenesis; Otic ves...

objective: the aim of this study was to evaluate the clinical efficacy of an herbal medicine, piascledine, on the treatment of chronic periodontitis. materials and methods: twenty-four patients with chronic periodontitis participated in this interventional case control investigation. after initial screening the subjects were divided into case and control groups. clinical measurements including ...

Aggrecanases from the ADAMTS (A Disintegrin And Metalloproteinase with ThromboSpondin motifs) family are important therapeutic targets due to their essential role in aggrecan depletion in arthritic diseases. Whether their function is also important for matrix rearrangements during chondrogenesis and thus, cartilage regeneration, is however so far unknown. The aim of this study was to analyse th...

One approach to resolving the complexities of chondrogenesis is to examine simplified systems in vitro. We analyzed cartilage differentiation by human adult stem cells from bone marrow stroma. Marrow stromal cells were cultured as micromass pellets for 21 days in serum-free medium containing transforming growth factor (TGF)-beta3, dexamethasone, and bone morphogenetic protein (BMP)-6. Assays fo...

BACKGROUND Hyaline cartilage tissue of joints is susceptible to injuries due to avascularity. Mesenchymal stem cells (MSCs) are used for cartilage tissue engineering. Among MSCs, adipose stem cells (ASCs) are attractive because of accessibility, their large number, and rapid growth. Common in vitro protocols successfully induce chondrogenic differentiation by expression of multiple cartilage-sp...

Bone morphogenetic protein 4 (BMP4) induces, whereas epidermal growth factor (EGF) inhibits chondrogenesis. We hypothesize that BMP4 and EGF mediated intracellular signals are both coupled in the regulation of Meckel's cartilage development. Two chondrogenic experimental model systems were employed to test the hypothesis: (1) an ex vivo, serum-free, organ culture system for mouse embryonic mand...