diosmin and hesperidin are natural flavonoid glycosides found in various plant materials, mainly in citrus fruits in different concentrations. diosmin for pharmaceutical use is obtained mainly semi-synthetically from hesperidin. hesperidin often accompanies diosmin as a natural impurity in different pharmaceutical formulations; therefore, a simple, fast and precise method for the simultaneous a...

an agar well diffusion bioassay method for determination of clarithromycin in human plasma, using micrococcus luteus atcc 9341 as the assay organism, was compared with a selective high-performance liquid chromatographic (hplc) method with uv detection. spiked plasma was used to prepare standard and control samples for both methods. the results of the bioassay analyses with spiked plasma samples...

carotenoids are red, orange and yellow pigments, which are widely distributed in fruit and vegetables. the aim of this research is to investigate the effect of conventional, high pressure and microwave cooking on the concentrations of β-carotene in carrot and sweet corn. raw carrots were washed, peeled and diced to a uniform size and then frozen at -70°c. sweet corn was also provided in a simil...

residual lignin of wood after mwl isolation was dissolved with 1-butyl-3-methylimidazolium chloride ionic liquid. chemical structure of isolated mwl from poplar wood (popolus deltiodes) and dissolved residual lignin were characterized with gas chromatography and 1d, 2d nmr techniques. analytical results showed that  popolus deltoides wood has more 4-(3-hydroxy-1-propenyl)-2-methoxy phenol units...

background & aim: according to the increase in diseases such as diabetes and obesity, using of saccharose alternatives for producing of low-calorie and sugar-free alternatives, are taken into consideration of researchers and industrialists. among them, steviosidewith the 300 times more sweetness than saccharose has special place, due to is a natural. the aim of this study is optimizing of stevi...

determination of drug residues in food is of great importance due to their toxicity. long-term exposure with low level of drug residues could be important, especially in children. based on document study, oxytetracycline (otc) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...

this study a novel high performance liquid chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using box–behnken experimental design. the method was performed in the isocratic mode on a rp-18 column (30 °c) using a mobile phase consisting of 0.05 m ammonium acetate buffer/ methanol mixture (30:70) and a flow-rate of 1.0 ml/min. the method was valida...

purpose : fluoroquinolones are widely used antibiotic for prophylaxis of intra and postoperative infections in individuals undergoing cataract surgery. this study was designed to assess the penetration of ciprofloxacin into the ocular aqueous humor (topical only versus topical and oral administration). methods : studied population (n=47) consisted of two groups: group one (n=26) and group two (...

separation of some biogenic amines via rp-hplc using mixed micellar mobile phase was investigated. the compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (sds and brij-35) and the ph of the mobile ...

in this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by hplc analysis. at first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (