introduction: fluorscence in situ hybridization (fish) enables specific detection of unique sequences of varying length, chromosomal regions or entire chromosomes within metaphase or interphase cells. recent developments in this technology permit the rapid mapping and ordering of dna fragments on single metaphase chromosome bands. the technique of fish incorporates several stages including: pro...

Two patients with acute blastic transformation of chronic myeloid leukemia (CML) associated with strikingly elevated platelet counts showed abnormalities of chromosome 3q in addition to the standard Philadelphia (Ph1) chromosome translocation. The first patient had an inversion of chromosome 3 (q21q26) cytologically identical to an inversion 3 previously reported in de novo acute megakaryoblast...

Hemopoietic cells in chronic granulocytic leukemia (CGL) frequently contain a chromosome translocation involving chromosome 22 and another autosome, usually number 9. The translocated chromosome 22 is known as the Philadelphia (Ph) chromosome. The appearance of a second Ph chromosome is the most common cytogenetic abnormality in CGL signaling the blastic phase. For 6 yr we serially studied a ma...

The 8;21 translocation is one of the most common specific rearrangements in acute myelogenous leukemia. We have identified markers (D21S65 and a Not I boundary clone, Not-42, referred to as probe B) flanking the chromosome 21 translocation breakpoint (21q22.3) that demonstrate physical linkage in normal genomic DNA, by using at least three restriction endonucleases (Not I, Sac II, and BssHII), ...

Chromosome segregation is fundamental to all cells, but the force-generating mechanisms underlying chromosome translocation in bacteria remain mysterious. Caulobacter crescentus utilizes a depolymerization-driven process in which a ParA protein structure elongates from the new cell pole, binds to a ParB-decorated chromosome, and then retracts via disassembly, pulling the chromosome across the c...

Background: Chronic myeloid leukemia (CML) is characterized by Philadelphia (Ph) chromosome. The Ph detected by karyotyping in 90% CML but 5-10% may have variant translocation where another chromosome addition to chromosome # 9 and 22. The aim of present report is to describe a rare three-way translocation with rarely describe 8p21 breakpoint. Case report: Retrospective cytogenetic database bet...

We report on a familial case including four male probands from three generations with a 45,X,psu dic(15;Y)(p11.2;q12) karyotype. 45,X is usually associated with a female phenotype and only rarely with maleness, due to translocation of small Y chromosomal fragments to autosomes. These male patients are commonly infertile because of missing azoospermia factor regions from the Y long arm. In our f...

Metaphase chromosomes of mice carrying Cattanach's translocation, which is the deletion of material from a medium-sized autosome and its insertion into an X chromosome, were stained with quinacrine mustard. Comparison of the fluorescence patterns of these chromosomes with those of the chromosomes of normal mice has allowed the identification of the autosome involved in the translocation and loc...

the highly conserved morphology and chromosomal structures of house mouse, mus musculus, provides a good model for chromosomal studies. sex-autosome translocations is one of the rarest chromosomal rearrangements among mammals and therefore sex-autosome translocations has been offered as accurate taxonomic markers to identify species with similar morphological traits. in this study, we described...

The chromosome breakpoints of a translocation, t(2;14), from an Epstein-Barr virus-transformed human B lymphoblastoid cell line were isolated and analyzed. This unusual translocation arose as a result of the fusion of two immunoglobulin (Ig) variable (V) genes, one from the heavy chain cluster on chromosome 14, the other from the light chain (k) cluster on chromosome 2. The chromosome breaks oc...