CLIP-170 is a microtubule 'plus end tracking' protein involved in several microtubule-dependent processes in interphase. At the onset of mitosis, CLIP-170 localizes to kinetochores, but at metaphase, it is no longer detectable at kinetochores. Although RNA interference (RNAi) experiments have suggested an essential role for CLIP-170 during mitosis, the molecular function of CLIP-170 in mitosis ...

CLIP-170 belongs to a group of proteins (+TIPs) with the enigmatic ability to dynamically track growing microtubule plus-ends. CLIP-170 regulates microtubule dynamics in vivo and has been implicated in cargo-microtubule interactions in vivo and in vitro. Though plus-end tracking likely has intimate connections to +TIP function, little is known about the mechanism(s) by which this dynamic locali...

The cytoplasmic linker protein (CLIP)-170, an outer kinetochore protein, has a role in kinetochore-microtubule attachment and chromosome alignment during mitosis. However, the mechanism by which CLIP-170 is involved in chromosome alignment is not known. Here, we show that CLIP-170 colocalizes with Polo-like kinase 1 (PLK1) at kinetochores during early mitosis. Depletion of CLIP-170 results in a...

Clip migration into the common bile duct (CBD) is a rare but well-established phenomenon of laparoscopic biliary surgery. The mechanism and exact incidence of clip migration are both poorly understood. Clip migration into the common bile duct can cause recurrent cholangitis and serve as a nidus for stone formation. We present a case, a 54-year-old woman, of clip-induced cholangitis resulting fr...

Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of IQGAP1, which includes ...

The possible occurrence of phosphorylation of proadrenocorticotropin(ACTH)/endorphin-derived peptides in the rat was examined by incubating dissociated anterior and intermediate pituitary cells with a %amino acid plus 32Pi. In both lobes of the pituitary newly synthesized pro-ACTH/endorphin was labeled with 32P. In the intermediate pituitary, corticotropin-like intermediate lobe peptide (CLIP) ...

An 86-year-old man with atrial fibrillation on anticoagulation was admitted to another hospital for an upper gastrointestinal bleed after accidental ingestion of a plastic bread clip. He required 11 units of packed red blood cells for resuscitation. Esophagogastroduodenoscopy demonstrated the clip firmly wedged onto a duodenal fold (Figure 1) with bleeding from adjacent mucosa. Attempts to remo...

CLiP (Code from Literate Program) is a tool which allows writing literate programs in virtually any programming language (Pascal, Fortran, C, C++, Assembler languages, etc) and in combination with almost any formatter (Runoff, Troff, TeX, LaTeX, etc.) or word-processor (Winword, Lotus Manuscript, Ami Pro, Word Perfect, Wordstar, etc). This posting explains the CLiP philosophy and compares it to...

Little is known about the distribution in normal cells of CLIP-170, a linkage mediator between endocytic vesicles and microtubules, and restin, a splice variant encoded by the same gene and marker for Hodgkin and Reed-Sternberg (HRS) cells of Hodgkin disease. Although only trace amounts of CLIP-170/restin are present in peripheral blood mononuclear cell subpopulations, monocyte-derived dendriti...

Dendritic arbors are compartments of neurons dedicated to receiving synaptic inputs. Their shape is an outcome of both the intrinsic genetic program and environmental signals. The microtubules and actin cytoskeleton are both crucial for proper dendritic morphology, but how they interact is unclear. The present study demonstrates that microtubule plus-end tracking protein CLIP-170 and actin-bind...