Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. The assay essentially tests every cell in the population for its ability to undergo ‘‘unlimited’’ division. Clonogenic assay is the method of choice to determine cell reproductive death after treatment w...

Sodium selenite supplementation at a concentration 50 nM before X-ray irradiation was suggested to protect non-cancerous human esophageal CHEK-1 cells from irradiation-induced damage. This present study investigated those effects on cancerous cell line. The cancer line, TE-8, cultured and supplemented for the cytotoxicity assay, GPx-1 activity, viability clonogenic assay western blot analysis. ...

PurposeThe in vitro clonogenic assay (IVCA) is the mainstay of quantitative radiobiology. Here, we investigate benefit a time-resolved IVCA version (trIVCA) to improve quantification survival and relative biological effectiveness (RBE) by analyzing cell colony growth behavior.Materials & MethodsIn IVCA, clonogenicity classification colonies performed based on fixed size threshold after incubati...

The neutral filter elution method and the neutral comet assay have been used to analyze radiation-induced DNA damage and repair in 6 human tumor cell lines: HT-144 melanoma; DU-145 prostate carcinoma; U-87 glioma; WiDr and HT-29 colon adenocarcinomas; and SiHa cervical carcinoma. In spite of large differences in intrinsic radiosensitivity measured using a clonogenic assay, double-strand break i...