the aim of this study was to determine the presence of toxin genes in clostridium perfringens isolated from 200minced meat samples using molecular typing. for this purpose, after isolation of clostridium perfringens using conventional culture method and confirmation by specific 16srd na gene pcr, alpha (cpa), beta (cpb), beta 2 (cpb2),epsilon (etx), iota (ia) and enterotoxin (cpe) genes were in...

Two dogs were diagnosed with enterotoxigenic Clostridium perfringens-associated diarrhea. Diarrhea was responsive to antimicrobial therapy, but recurred after treatment was ceased. Clostridium perfringens enterotoxin was present in feces during diarrheic episodes but not when feces were normal. Both dogs responded to a prolonged course of oral cephalexin and dietary modification.

Di-tri-octahedral smectite was effective in neutralizing Clostridium difficile toxins A and B as well as Clostridium perfringens enterotoxin in vitro. However, di-tri-octahedral smectite did not inhibit the growth of Clostridium difficile and Clostridium perfringens, nor did it interfere with the activity of metronidazole. Di-tri-octahedral smectite may be a useful option for the treatment of c...

Antagonistic effect of piglet microflora against Clostridium perfringens was studied in germfree mice, to isolate bacterial strains responsible for this colonization resistance. The 1:100 dilution of the feces of a 2 day-old conventional piglet, given per os to germfree mice already harboring C. perfringens, led to the elimination of C. perfringens. From this piglet flora, 8 bacterial strains w...

clostridial myositis, considered to be rare in pet animals, is an acutely fatal toxaemic condition. some species of clostridia are responsible for necrotic myositis. a 2-year-old male german shepherd dog was admitted with non-weight bearing lameness and massive swelling of the left hind limb. clostridium perfringens type a with alpha toxin was diagnosed as a pathogenic agent. based on the histo...

The present investigation was conducted to study the effects of experimental Clostridium perfringens type D enterotoxaemia in teddy goats. Clinical signs started to appear after 30 min of experimental infection like anorexia, diarrhea, dehydration, frothing and dyspnea. Gross lesions consisted of severe congestion in tissues of varying intensity with enlarged mesenteric lymph nodes while histol...

background and objectives: clostridium perfringens is more prevalent type of clostridia genus isolated from the intestinal tract of ostrich ( struthio camelus ). necrotic enteritis (ne) is a potentially fatal gastrointestinal (gi) disease of poultry and other avian species, which produces marked destruction of intestinal lining in digestive tract caused by c. perfringens . pathogenicity and les...

background : clostridium perfringens type c strains cause severe necrotizing enteritis in sheep, calves, goats, pigs and humans. beta-toxin is introduced to be the essential virulence factor of this microorganism. in the present study, a new method was established for the purification of beta toxin from culture supernatant fluid of c. perfringens type c vaccinal strain. methods : the four steps...

Clostridium perfringens strain LLY_N11, a commensal bacterium, which previously induced necrotic enteritis in an experimental study, was isolated from the intestine of a young healthy chicken. Here, we present the complete genome sequence of this strain, which may provide a better understanding of the molecular mechanisms involved in necrotic enteritis pathogenesis.