A method was developed for extracting enzymes from micro-organisms closely associated with ammonia-treated straw (NH3-S) that had been incubated in nylon bags in the rumen. Incubation of washed straw with 125 ml carbon tetrachloride/l and 20 micrograms lysozyme/ml for 3 h at 37 degrees gave carboxymethylcellulase (EC 3.2.1.4; CMCase) and NAD-linked glutamate dehydrogenase (EC 1.4.1.2; GDH) acti...

Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass. Out of total 80 microbial isolates from different ecological niches one bacterial strain, identified as Bacillus sp. 313SI, was selected for CMCase production under stationary as well as shaking conditions of growth. During two-stage pretreatment, rice str...

Cellulolytic enzymes produced by spore-forming bacteria seem to be a potential solution the degradation of lignocellulosic waste. In this study, several dozen bacterial strains were isolated from soil and one them was selected for further studies. The studied strain identified genus Bacillus (strain 8E1A) 16S rRNA gene sequencing. sp. 8E1A showed an activity carboxymethyl cellulase (CMCase) wit...

Cellulase-producing fungi Trichoderma viride were cultured and fermented on the solid-state wheat bran fermentation medium. The characteristics of its carboxymethyl cellulase (CMCase) in the condition of this solid-state fermentation were evaluated, and the optimum culture time, optimum pH and optimum temperature for CMCase activity of T. viride fermented in this solid state were 60h, 5.0 and 5...

Intergeneric protoplast fusion between Escherichia coli HB101 with pBR322 carrying the cloned o-(carboxymethyl)cellulase (CMCase) gene of Ruminococcus albus (Pro Leu Ap Km) and an anaerobic mutant strain, FEM29 (Trp His Ap Km), with dehydrodivanillin-degrading activity was performed in the presence of 40% polyvinyl alcohol 300 under aerobic and anaerobic conditions to transfer the cloned cellul...

A Bacillus isolate AK5 which was provisionally identified as Bacillus brevis isolated from the Acacia forest soil at University of Chittagong was found to be capable of producing cellulolytic enzymes during growth on different cellulosic substrates. The isolate AK5 grown under different conditions showed that CMC was the best cellulosic substrates for inducing the synthesis of extracellular cel...

A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on S...

The present work deals with the development of solid state fermenter for enzymes production and the development of a pilot scale of enzymatic deinking system. The newly developed solid state fermenter, was named FERMSOSTAT®, which was used for the production of cellulases and xylanase using a local isolate; Aspergillus niger USM AI 1. Optimization of enzymes production was carried out and the o...

Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. Among three potential bacteria examined, Bacillus licheni...

Cellulase production was investigated in pH-controlled cultures of Acremonium cellulolyticus. The response to culture pH was investigated for three cellulolytic enzymes, carbomethyl cellulase (CMCase), avicelase, and beta-glucosidase. Avicelase and beta-glucosidase showed similar profiles, with maximum activity in cultures at pH 5.5-6. The CMCase activity was highest in a pH 4 culture. At an ac...