Heat treatment of milk causes the heat-denaturable whey proteins to aggregate with kappa-casein (kappa-CN) via thiol-disulfide bond interchange reactions. The particular disulfide bonds that are important in the aggregates are uncertain, although Cys(121) of beta-lactoglobulin (beta-LG) has been implicated. The reaction at 60 degrees C between beta-LG A and an activated kappa-CN formed small di...

Direct and straightforward methods to follow nucleic acid cleavage are needed. A spectrophotometric quadruplex formation assay (QFA) was developed, which allows real-time monitoring of site-specific cleavage of nucleic acids. QFA was applied to study both protein and nucleic acid restriction enzymes, and was demonstrated to accurately determine Michaelis-Menten parameters for the cleavage react...

The involvement of chemical exchange in 2D IR heterodyne echo spectroscopy is characterized through the hydrogen-bond exchange between CH3OH and the CN of CH3CN. The exchange dynamics on the hydrogen-bond potential surfaces associated with different quantum states of the high-frequency CN stretching mode contributes to strong cross peaks between CN groups in two different chemical configuration...

in this work, the study of various substituted kaempferol derivatives is presented. the bond dissociation energies (bde) have been calculated using b3lyp level and different basis sets in gas-phase. calculated results show that the bde values of substituted kaempferol range from about 77 to100 kcal/mol, demonstrating that kaempferol is an effective chain-breaking antioxidant that prevents lipid...

This review covers H–H bond cleavage of dihydrogen (H2) mediated by structurally well-defined rare-earth metal (scandium, yttrium and lanthanides) complexes, their applications in homogenous catalysis, such as catalytic hydrogenation unsaturated organic molecules. Depending on the mechanism cleavage, this is organized two parts: (1) ?-bond metathesis, (2) non-?-metathesis H2 activation. The lat...

The preparation and characterization of three new paramagnetic complexes of the 17-electron Re(II) ion are reported. The salts [Re(triphos)(CH(3)CN)(3))][X](2), X = [BF(4)](-) (1), [PF(6)](-) (2), and [Et(4)N][Re(triphos)(CN)(3)] (3) were prepared by homolytic cleavage of the Re-Re bond in [Re(2)(CH(3)CN)(10)][BF(4)](4) or by disruption of the chlorine bridges in [(triphos)Re(mu-Cl)(3)Re(tripho...

In this work, the study of various substituted Kaempferol derivatives is presented. The bond dissociation energies (BDE) have been calculated using B3LYP level and different basis sets in gas-phase. Calculated results show that the BDE values of substituted Kaempferol range from about 77 to100 kcal/mol, demonstrating that Kaempferol is an effective chain-breaking antioxidant that prevents lipid...

Calcineurin, a Ca(2+)/calmodulin-dependent Ser/Thr phosphatase (protein phosphatase 2B), plays a critical role in IL-2 production during T cell activation. It has been previously reported that IL-2 release in activated Jurkat T requires caspase-like activity (Posmantur et al. (1998) Exp. Cell. Res. 244, 302-309). We report here that the 60-kDa catalytic subunit of calcineurin A (Cn A) was parti...

Abstract The intermolecular three-component alkene vicinal dicarbofunctionalization (DCF) reaction allows installation of two different carbon fragments. Despite extensive investigation into its ionic chemistry, the enantioseletive radical-mediated versions DCF reactions remain largely unexplored. Herein, we report an intermolecular, enantioselective radical olefins enabled by merger addition a...

In this study, the reactions of electrons with N-acetylproline are investigated by electron spin resonance (ESR) spectroscopy and density functional theory. Electrons are produced by γ irradiation or by photoionization of K(4)Fe(CN)(6) in neutral 7.5 M LiCl-D(2)O aqueous glasses at low temperatures with identical results. Electrons are found to add to both the peptide bond and the carboxyl grou...