A cDNA encoding an Na+-Pi co-transporter, termed rat PiT-1, has now been isolated from rat parathyroid. Expression of rat PiT-1 in Xenopus oocytes revealed that it possesses Na+-dependent Pi co-transport activity. The amount of PiT-1 mRNA in the parathyroid of vitamin D-deficient rats was reduced compared with that in normal animals, and increased markedly after administration of 1,25-dihydroxy...

This paper presents a new 4-degree-of-freedom parallel mechanism dedicated to handling of heavy parts within a large workspace. These 4 degrees of freedom are 3 translations and 1 rotation about a given axis. Such a mechanism is rare; most of common parallel mechanisms have 3 or 6 degrees of freedom. Firstly, a description of the mechanism is given. Then models are derived regarding velocity an...

We present a combined experimental and theoretical study on the radiative lifetime of CO in the a (3)Pi(1,2), v=0 state. CO molecules in a beam are prepared in selected rotational levels of this metastable state, Stark-decelerated, and electrostatically trapped. From the phosphorescence decay in the trap, the radiative lifetime is measured to be 2.63+/-0.03 ms for the a (3)Pi(1), v=0, J=1 level...

Isolated spinach chloroplasts purified by isopycnic centrifugation in density gradients of Percoll were found to be highly intact, to be devoid of extrachloroplastic contaminations, and to retain a high rate of CO(2)-dependent O(2) evolution.When suspended in a medium which avoided rupture of the envelope, intact purified chloroplasts progressively lost their phosphate content by passive diffus...

Oxygen conversion process between O₂ and H₂O by means of electrochemistry or photochemistry has lately received a great deal of attention. Cobalt-phosphate (Co-Pi) catalyst is a new type of cost-effective artificial oxygen-evolving complex (OEC) with amorphous features during photosynthesis. However, can such Co-Pi OEC also act as oxygen reduction reaction (ORR) catalyst in electrochemical proc...

During the process of matrix vesicle (MV)-mediated initiation of mineralisation, chondrocytes and osteoblasts mineralise the extracellular matrix by promoting the seeding of basic calcium phosphate crystals of hydroxyapatite (HA) along the collagen fibrils. This orchestrated process is carefully regulated by the balanced action of propagators and inhibitors of calcification. The primary antagon...

Date of Report: July 2010 EPA Agreement Number: RD-83283501 Title: An Instrument for Real Time Speciation of Water Soluble Tracers in Atmospheric Particulate Matter Investigators: Rodney J. Weber (PI Georgia Tech), James J. Schauer (Co-PI, UWMadison), Martin Shafer (Co-PI, UW-Madison) Institution: Georgia Institute of Technology Research Category: Continuous Measurement Methods For Particulate ...

Phosphatidylinositol (PI) 3-kinase plays an important role in the signalling of cell growth. We previously purified two types of PI 3-kinase from bovine thymus, a monomer from (PI 3-kinase I) and a heterodimer form (PI 3-kinase II) [Shibasaki, Homma and Takenawa (1991) J. Biol. Chem. 266, 8108-8114]. Here we examine the properties of these purified PI 3-kinases. Both PI 3-kinases were inhibited...

We report magnetic and electrical measurements of Nb Josephson junctions with strongly ferromagnetic barriers of Co, Ni, and Ni80Fe20 (Py). All these materials show multiple oscillations of critical current with a barrier thickness implying repeated 0-pi phase transitions in the superconducting order parameter. We show, in particular, that the Co barrier devices can be accurately modeled using ...

The pulvinar of primates, which connects with all visual areas, has been implicated in visual attention and in control of eye movements. Recently, five separate neurochemical subdivisions of a region termed the inferior pulvinar complex have been identified in monkeys (Gray et al. [1999] J Comp Neurol 409:452-468; Gutierrez et al. [1995] J Comp Neurol 363:545-562), and comparable subdivisions h...