Exploration of pathogenic potential Coagulase-Negative Staphylococci (CoNS), in human as well animals,gained the importance during past two decades. Emergence Methicillin-Resistant Coagulase-NegativeStaphylococci (MRCoNS) bovine milk poses to be a major public health concern. Presence mecA gene isone most common reasons reported for methicillin resistant bacteria. Since scientific data concerni...

BBL ™ CHROMagar ™ MRSA (CMRSA) is a selective and differential medium designed for the direct detection of methicillin-resistant Staphylococcus aureus (MRSA) from nares specimens. CMRSA incorporates specific chromogenic substrates and cefoxitin to facilitate the detection of MRSA. Identification is considered definitive following 24 h incubation; however, confirmatory testing, such as coagulase...

The objective of this study was to evaluate the prevalence of coagulase-negative staphylococci in healthy dogs and to determine whether methicillin-resistant staphylococci expressed the mecA gene. Nasal and rectal swab samples were taken from 50 clinically healthy dogs. The prevalence of coagulase-negative staphylococci was evaluated according to phenotypic properties. The agar diffusion method...

The close correlation between the ability of coagulase to clot blood plasma and their capacity to produce disease, and the corresponding absence of this property in nonpathogenic strains, have led to the assumption that the coagulase, plays important role in the pathogenesis of disease. Currently, crystal structure of coagulase in Staphylococcus aureus remains indefinable. Thus, the objectives ...

Staphylococci involve infections in association with a number of bacterial virulence factors. Extracellular enzymes play an important role in staphylococcal pathogenesis. In addition, biofilm is known to be associated with their virulence. In this study, 149 staphylococcal isolates from acne lesions were investigated for their virulence factors including lipase, protease, and biofilm formation....

Eight cephalosporins were tested for their activity against methicillin-susceptible and methicillin-resistant, coagulase-negative staphylococci and for their resistance to beta-lactamase from methicillin-resistant, coagulase-negative staphylococci. Susceptibility testing by the agar plate method was evaluated for the effect of inoculum size and duration of incubation. Methicillin-susceptible, c...

Of the methods now available for the detection of coagulase positive staphylococei, none is designed to show both clumping factor and free coagulase activity in a single system. The observations reported here are by-products of a search for variant strains of staphylococci with particular reference to strain differences in the cell surface component known as clumping factor or bound coagulase. ...

Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, are increasingly important causes of nosocomial infection. Microbiologists and clinicians no longer can afford to disregard clinical isolates of coagulase-negative staphylococci as contaminants. Accurate species identification and antimicrobial susceptibility testing, in a clinically relevant time frame, are important ai...

Of 700 coagulase-positive staphylococcal strains isolated from humans, 692 (98.9%) produced protein A. Of 100 coagulase-negative strains, 2 were protein A-positive.

OBJECTIVE To determine the frequency of isolation of coagulase-negative staphylococci and their resistance to methicillin over a period of time. METHODS The descriptive cross-sectional study was carried out at Army Medical College, Rawalpindi, from June 2009 to May 2012, and comprised clinical samples mostly from patients admitted to the intensive care unit. They were inoculated onto appropri...